Effect of gonadectomy and steroid treatment on the receptor-binding activity and immunoreactivity of serum and pituitary FSH in the adult rats of both sexes.
Effect of gonadectomy and steroid treatment on the receptor-binding activity and immunoreactivity of serum and pituitary FSH in the adult rats of both sexes.
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性腺切除和类固醇治疗对两性成年大鼠血清和垂体 FSH 受体结合活性和免疫反应性的影响。
DOI：
10.1507/endocrj1954.28.347
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Wakabayashi
中科院分区：
文献类型：
--
作者：
T. Minegishi;M. Igarashi;K. Wakabayashi
采用放射受体分析法和放射免疫分析法分别测定了正常、性腺切除和性腺激素治疗的雄性和雌性大鼠血清和垂体FSH的受体结合活性（R）和免疫反应性（I），并分析了R/I比值。在11周龄时，血清和垂体FSH的R/I比值在正常女性中显著高于男性。11 ~ 13周龄，雌性血清FSH和垂体FSH比值进一步升高，雄性血清FSH比值降低，垂体FSH比值升高。睾丸切除导致血清FSH比率增加，而卵巢切除导致血清FSH比率降低，因此两种性别的性腺切除动物的比率几乎相等，表明这些动物分泌性腺切除型FSH。丙酸睾丸酮（TP）和苯甲酸雌二醇（EB）治疗均降低去性腺大鼠血清FSH比值。而TP和EB对垂体FSH比值的影响则相反，TP使垂体FSH比值降低，EB使垂体FSH比值升高。这种影响在女性中比男性更明显。这些观察结果表明，性腺的存在和缺乏，以及性腺类固醇激素不仅影响FSH的数量，而且影响FSH的质量。在激素的质量的变化进行了讨论，其多样性。
The receptor binding activity (R) and immunoreactivity (I) of serum and pituitary FSH were measured in normal, gonadectomized, and gonadal steroid-treated rats of both sexes employing radioreceptor assay and radioimmunoassay, respectively, and R/I ratios were analyzed. At 11 weeks of age, R/I ratios of serum and pituitary FSH were significantly higher in normal females than in males. From 11 to 13 weeks of age, the ratios of both FSH further increased in females, while in males, the ratio of serum FSH decreased and that of pituitary FSH increased. Orchiectomy caused an increase in the ratio of serum FSH, while ovariectomy caused a decrease, so the ratios in the gonadectomized animals of both sexes became almost equal, suggesting the secretion of a gonadectomy type FSH in these animals. Both testosterone propionate (TP) and estradiol benzoate (EB) treatments decreased the ratios of serum FSH in gonadectomized rats. However, TP and EB had an effect opposite on the ratio of pituitary FSH in these rats, i.e. TP caused a decrease, while EB caused an increase. This effect was more obvious in females than in males. These observations indicated that the presence and absence of the gonads, and the gonadal steroids influence not only the quantity but also the quality of FSH. The changes in the quality of the hormone are discussed in relation to its multiplicity.