Variability in hepatic iron concentration in percutaneous needle biopsy specimens from patients with transfusional hemosiderosis
Variability in hepatic iron concentration in percutaneous needle biopsy specimens from patients with transfusional hemosiderosis
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DOI：
10.1309/puuxegxdlh26nxa2
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发表时间：
2005-01-01
影响因子：
3.5
通讯作者：
Harmatz, P
中科院分区：
文献类型：
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作者：
Butensky, E;Fischer, R;Harmatz, P
在长期接受红细胞输注的镰状细胞病或β地中海贫血患者中，准确了解肝铁浓度(LIC)对于治疗是至关重要的。2例患者行LIC和肝脏病理检查，重复活检2次，取自同一肝脏部位。分析了无微量元素容器中的新鲜组织核心和溶解的石蜡包埋核心的组织。两个石蜡包埋的核的LIC测量结果没有显著差异(中位数为12,455比12,153微克/克干重；n=29)。当对1个新鲜组织样本和1个石蜡包埋的核进行分析时，发现有显著差异(中位数，11,716比12,864µg/g干重；n=16；P<；.001)，两种LIC测量之间的中位数不一致为23.0%。我们发现在……中有很高的同意性。石蜡包埋技术处理的肝活检标本之间的LICs，但与干燥的新鲜组织样本相比，LICs被高估。
In patients with sickle cell disease or beta-thalassemia receiving RBC transfusions for a long period a precise knowledge of the liver iron concentration (LIC) is essential for treatment. Patients underwent LIC and liver pathology assessment by duplicate biopsies in 2 passes from the same local liver site. Fresh tissue cores in trace element free containers and tissues from dissolved paraffin-embedded cores were analyzed. LIC measurements in each of 2 paraffin-embedded cores did not differ significantly (median, 12,455 vs 12,153 mug/g dry weight; n = 29). A significant difference was observed when 1 fresh tissue sample and 1 paraffin-embedded core were analyzed (median, 11,716 vs 12, 864 mug/g dry weight; n = 16; P < .001) with a median disagreement between LIC measurements of 23.0%. We found high agreement in. LICs between liver biopsy specimens processed by the paraffin-embedding technique but overestimation of LICs in comparison with desiccated fresh tissue samples.