Getting to the root of plant biology: impact of the Arabidopsis genome sequence on root research.
Getting to the root of plant biology: impact of the Arabidopsis genome sequence on root research.
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DOI：
10.1111/j.1365-313x.2010.04129.x
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发表时间：
2010-03
期刊：
影响因子：
--
通讯作者：
Schiefelbein J
中科院分区：
文献类型：
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作者：
Benfey PN;Bennett M;Schiefelbein J
在基因组序列出现之前，拟南芥的根吸引了一小群热情的研究人员，他们被它的可及性和发育的简单性所吸引。当根生长在营养琼脂介质的表面时，很容易观察到根，这有助于分析对重力和触摸等刺激的反应。发育生物学家被初级根组织的简单放射状组织所吸引，这些组织在中央维管组织周围形成一系列同心圆柱体。同样吸引人的是繁殖模式，顶端的干细胞产生的后代被限制在细胞档案中。根发育的这些特性将正常的四维发育问题(三维空间和时间)简化为二维问题，细胞类型在径向轴上，发育时间在纵轴上。拟南芥全基因组序列的获得极大地加速了传统的根生物学遗传学研究，也使全新的实验策略得以应用。在这里，我们回顾了拟南芥基因组序列的可获得性促进了对根生物学的理解的进展的方法的例子。
Prior to the availability of the genome sequence, the root of Arabidopsis had attracted a small but ardent group of researchers drawn to its accessibility and developmental simplicity. Roots are easily observed when grown on the surface of nutrient agar media, facilitating analysis of responses to stimuli such as gravity and touch. Developmental biologists were attracted to the simple radial organization of primary root tissues, which form a series of concentric cylinders around the central vascular tissue. Equally attractive was the mode of propagation, with stem cells at the tip giving rise to progeny that were confined to cell files. These properties of root development reduced the normal four-dimensional problem of development (three spatial dimensions and time) to a two-dimensional problem, with cell type on the radial axis and developmental time along the longitudinal axis. The availability of the complete Arabidopsis genome sequence has dramatically accelerated traditional genetic research on root biology, and has also enabled entirely new experimental strategies to be applied. Here we review examples of the ways in which availability of the Arabidopsis genome sequence has enhanced progress in understanding root biology.