Analysis of equine humoral immune responses to the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus.
Analysis of equine humoral immune responses to the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus.
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马传染性贫血病毒跨膜包膜糖蛋白（gp45）的马体液免疫反应分析。
DOI：
10.1128/jvi.65.2.1013-1018.1991
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发表时间：
1991
影响因子：
5.4
通讯作者：
Rushlow,KE
中科院分区：
文献类型：
--
作者：
Chong,YH;Ball,JM;Issel,CJ;Montelaro,RC;Rushlow,KE
马传染性贫血病毒跨膜包膜糖蛋白（gp45）的特定片段表达为TrpLE融合蛋白，并在Western免疫印迹中检测其对多种马免疫血清的反应性。gp45最具免疫原性的区域定位于其氨基端，位于疏水融合和跨膜结构域之间。一系列重叠的合成肽用于酶联免疫吸附测定，以确定该区域内的免疫显性表位。相比之下，gp45的羧基末端对马免疫血清表现出弱且可变的免疫反应性。
Defined segments of the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus were expressed as TrpLE fusion proteins and examined for their reactivity in Western immunoblots against a diverse panel of equine immune sera. The most immunogenic region of gp45 was localized to its amino terminus, positioned between the hydrophobic fusion and the transmembrane domains. A series of overlapping synthetic peptides were used in enzyme-linked immunosorbent assays to define an immunodominant epitope within this region. In contrast, the carboxy-terminal half of gp45 displayed both weak and variable immunoreactivity with equine immune sera.