Induction of the Candida albicans filamentous growth program by relief of transcriptional repression:: A genome-wide analysis
Induction of the Candida albicans filamentous growth program by relief of transcriptional repression:: A genome-wide analysis
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DOI：
10.1091/mbc.e05-01-0073
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发表时间：
2005-06-01
影响因子：
3.3
通讯作者：
Johnson, AD
中科院分区：
文献类型：
--
作者：
Kadosh, D;Johnson, AD
白色念珠菌是主要的人类真菌病原体，经历从芽生孢子（圆形出芽细胞）到丝状体（首尾相连的细长细胞）的可逆形态转变。这种转变是在白色念珠菌细胞暴露于多种宿主条件（包括血清和体温（37℃））时诱导的，是毒力所必需的。使用全基因组 DNA 微阵列分析，我们描述了 61 个基因，这些基因在胚芽孢子到丝状体的转变过程中被显着诱导（> = 2 倍），这种转变发生在暴露于血清和 37 摄氏度的情况下。接下来，我们表明，这些基因中大约一半在胚芽孢子状态下受到三种转录抑制因子 Rfg1、Nrg1 和 Tup1 的转录抑制。我们得出的结论是，这种转录抑制的缓解在使白色念珠菌丝状生长程序发挥作用方面发挥着关键作用，并且我们描述了这种转录回路的框架。
Candida albicans, the major human fungal pathogen, undergoes a reversible morphological transition from blastospores (round budding cells) to filaments (elongated cells attached end-to-end). This transition, which is induced upon exposure of C. albicans cells to a number of host conditions, including serum and body temperature (37 degrees C), is required for virulence. Using whole-genome DNA microarray analysis, we describe 61 genes that are significantly induced (>= 2-fold) during the blastospore to filament transition that takes place in response to exposure to serum and 37 degrees C. We next show that approximately half of these genes are transcriptionally repressed in the blastospore state by three transcriptional repressors, Rfg1, Nrg1, and Tup1. We conclude that the relief of this transcriptional repression plays a key role in bringing the C. albicans filamentous growth program into play, and we describe the framework of this transcriptional circuit.