Studies on the essential role of ascorbic acid in the energy dependent release of insulin from pancreatic islets
Studies on the essential role of ascorbic acid in the energy dependent release of insulin from pancreatic islets
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DOI：
10.1006/bbrc.1997.6106
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发表时间：
1997-02-24
影响因子：
3.1
通讯作者：
Wells, WW
中科院分区：
文献类型：
--
作者：
Dou, CZ;Xu, DP;Wells, WW
用去极化水平的KCl（45 mM）和20 mM n-甘油醛刺激年轻正常和坏血病雄性豚鼠的胰岛，检测它们释放胰岛素的能力。正常豚鼠的胰岛以K+和D-甘油醛依赖性成熟释放胰岛素，显示快速的初始分泌相，随后在120 min内出现胰岛素释放的第二波。从坏血病豚鼠的胰岛能够响应的方式相同的控制胰岛的高K+，相反，胰岛素释放从抗坏血酸缺乏胰岛的促分泌素，D-甘油醛，显着延迟和减少的反应，在120分钟期间观察到D-甘油醛刺激后。结果与抗坏血酸对能量依赖性胰岛素释放的作用位点一致，该位点位于糖酵解的磷酸丙糖水平和氧化磷酸化产生ATP之间。(C)北京：科学出版社.
Pancreatic islets from young normal and scorbutic male guinea pigs were examined for their ability to release insulin when stimulated with depolarizing levels of KCI (45 mM) and by 20 mM n-glyceraldehyde, Islets from normal guinea pigs released insulin in a K+ and D-glyceraldehyde dependent maturer showing a rapid initial secretion phase followed by secondary waves of insulin release during a 120 min period. Islets from scorbutic guinea pigs were able to respond to elevated K+ in a manner identical to that of the control islets, In contrast, insulin release from ascorbic acid deficient islets in response to the secretagogue, D-glyceraldehyde, was significantly delayed and decreased responses were observed during the 120 min period after D-glyceraldehyde stimulation. The results are consistent with the site of action of ascorbic acid on energy-dependent insulin release lying between the triose-phosphate level of glycolysis and the generation of ATP by oxidative phosphorylation. (C) 1997 Academic Press.