A tissue engineering strategy for the treatment of avascular necrosis of the femoral head.
A tissue engineering strategy for the treatment of avascular necrosis of the femoral head.
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DOI：
10.1016/j.surge.2013.02.008
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发表时间：
2013-12
影响因子：
2.5
通讯作者：
Oreffo, R. O. C.
中科院分区：
文献类型：
--
作者：
Aarvold, A.;Smith, J. O.;Tayton, E. R.;Jones, A. M. H.;Dawson, J. I.;Lanham, S.;Briscoe, A.;Dunlop, D. G.;Oreffo, R. O. C.
骨干细胞（SSC）和冲击骨移植（IBG）可以组合以产生机械稳定的活骨复合材料。这种新的策略已被用于治疗股骨头缺血性坏死。介绍了该翻译病例系列的手术技术、临床随访和检索分析数据。4例患者的5个股骨头中，将SSC和研磨的同种异体移植物打入坏死骨中。通过细胞移植物构建体的平行体外培养证实细胞活力。通过系列临床和放射学检查对患者进行随访。从两个取出的股骨头中取出组织工程骨，并通过组织学、显微计算机断层扫描（μCT）和力学测试进行分析。3例患者在22至44个月随访时仍无症状。1例患者（双侧髋关节）因广泛残留坏死需要全髋关节置换术。取出的组织工程骨在组织学和μCT上显示成熟的小梁微结构。骨密度和轴向压缩强度与骨小梁相当。临床随访表明，该方法是治疗早期局灶性股骨头缺血性坏死的有效新方法。临床转化的组织工程骨的独特回收分析已经证明组织再生在结构和功能上都类似于正常的骨小梁。
Skeletal stem cells (SSCs) and impaction bone grafting (IBG) can be combined to produce a mechanically stable living bone composite. This novel strategy has been translated to the treatment of avascular necrosis of the femoral head. Surgical technique, clinical follow-up and retrieval analysis data of this translational case series is presented. SSCs and milled allograft were impacted into necrotic bone in five femoral heads of four patients. Cell viability was confirmed by parallel in vitro culture of the cell-graft constructs. Patient follow-up was by serial clinical and radiological examination. Tissue engineered bone was retrieved from two retrieved femoral heads and was analysed by histology, microcomputed tomography (μCT) and mechanical testing. Three patients remain asymptomatic at 22- to 44-month follow-up. One patient (both hips) required total hip replacement due to widespread residual necrosis. Retrieved tissue engineered bone demonstrated a mature trabecular micro-architecture histologically and on μCT. Bone density and axial compression strength were comparable to trabecular bone. Clinical follow-up shows this to be an effective new treatment for focal early stage avascular necrosis of the femoral head. Unique retrieval analysis of clinically translated tissue engineered bone has demonstrated regeneration of tissue that is both structurally and functionally analogous to normal trabecular bone.
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影响因子：
1
作者：
Hernigou P;Poignard A;Zilber S;Rouard H
通讯作者：
Rouard H
DOI：
10.1302/0301-620x.89b3.18080
发表时间：
2007-03-01
影响因子：
--
作者：
van Haaren, E. H.;Heyligers, I. C.;Wuisman, P. I. J. M.
通讯作者：
Wuisman, P. I. J. M.
影响因子：
2.7
作者：
Wang, Bai-Liang;Sun, Wei;Li, Zi-Rong
通讯作者：
Li, Zi-Rong
DOI：
10.1302/0301-620x.88b4.16950
发表时间：
2006-04-01
影响因子：
--
作者：
Keizer, SB;Kock, NB;Nelissen, RGHH
通讯作者：
Nelissen, RGHH
DOI：
10.1097/00003086-199603000-00020
发表时间：
1996-03-01
影响因子：
4.2
作者：
Mont, MA;Carbone, JJ;Fairbank, AC
通讯作者：
Fairbank, AC