Analysis of 2-Methylisoborneol in Water, Mud, and Channel Catfish (Ictalurus punctatus) from Commercial Culture Ponds in Mississippi
Analysis of 2-Methylisoborneol in Water, Mud, and Channel Catfish (Ictalurus punctatus) from Commercial Culture Ponds in Mississippi
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密西西比州商业养殖池塘中水、泥和斑点叉尾鮰 (Ictalurus punctatus) 中 2-甲基异龙脑的分析
DOI：
10.1139/f87-109
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
L. Bennett
中科院分区：
文献类型：
--
作者：
J. Martín;C. P. McCoy;W. Greenleaf;L. Bennett
介绍了一种从已被宣布为“异味”的斑点叉尾鱼中分离2-甲基异龙脑(MIB)的新方法。该方法是在氮气气流下微波煮鱼，在−80°C捕集冷凝液，用正己烷提取鱼冷凝液，还原到100 μL，用气相色谱分析。常规检测低至5 ng MIB∙g FISH−1。异味鱼肉中MIB的生物浓缩值为28.1MIB± 14.0(±SD)水/浓缩鱼。对加工厂收集的异味鱼的分析始终显示MIB浓度升高。另一种已知会导致鱼肉风味和气味问题的化合物Geosmin没有被检测到。
We describe a new method for the isolation of 2-methylisoborneol (MIB) from channel catfish (Ictalurus punctatus) that have been declared "off-flavor" organoleptically. The method involves microwave cooking fish under a nitrogen stream and trapping the condensate at −80 °C. The fish condensate was extracted with hexane which was reduced to 100 μL and analyzed by gas chromatography. Concentrations as low as 5 ng MIB∙g fish−1 were routine detected. A bioconcentration value for MIB in off-flavored fish flesh was 28.1 ± 14.0 (±SD) concentration water/concentration fish. Analysis of off-flavored fish collected at the processing plant consistently showed elevated concentrations of MIB. Geosmin, another compound known to cause flavor and odor problems in fish flesh, was not detected.