OXIDATION OF PROLINE BY PLANT-MITOCHONDRIA
OXIDATION OF PROLINE BY PLANT-MITOCHONDRIA
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DOI：
10.1104/pp.62.1.22
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发表时间：
1978-01-01
期刊：
影响因子：
7.4
通讯作者：
STEWART, CR
中科院分区：
文献类型：
--
作者：
BOGGESS, SF;KOEPPE, DE;STEWART, CR
从玉米(Zea Mays)、小麦(Triticum Aestivum)、大麦(Hordeum Uggare)、大豆(Glyine max L.Merr.)绿豆(Phaseolus Aureus)表现出依赖于脯氨酸的氧气吸收，受呼吸控制。以脯氨酸为底物的ADP/O介于外源NADH和苹果酸+丙酮酸为底物的ADP/O之间。同位素研究表明，脯氨酸代谢依赖于O2，而不是NAD。DELTA1-吡咯烷-5-羧酸生成的主要吲哚三酮阳性产物为谷氨酸。线粒体能够进一步代谢谷氨酸，放射性二氧化碳、有机酸和天冬氨酸在[14C]补饲实验后均恢复正常。证明了植物脯氨酸代谢的线粒体结合和氧依赖性。
Mitochondria isolated from etiolated shoots of corn (Zea mays), wheat (Triticum aestivum), barley (Hordeum vulgare), soybean (Glycine max L. Merr.) and mung bean (Phaseolus aureus) exhibited a proline-dependent O2 uptake subject to respiratory control. ADP/O ratios with proline as substrate were intermediate between ratios obtained with exogenous NADH and malate + pyruvate as substrates. Isotope studies showed proline metabolism to be dependent on O2, but not NAD. The major ninhydrin-positive product formed via .DELTA.1-pyrroline-5-carboxylic acid was glutamate. Mitochondria were capable of further metabolism of glutamate, as radioactive CO2, organic acids and aspartate were recovered after [14C]proline feeding experiments. The mitochondrial association and O2 dependence of plant proline metabolism were demonstrated.