Evidence against direct involvement of cyclic GMP or cyclic AMP in bacterial chemotactic signaling.
Evidence against direct involvement of cyclic GMP or cyclic AMP in bacterial chemotactic signaling.
复制标题
反对环 GMP 或环 AMP 直接参与细菌趋化信号传导的证据。
DOI：
10.1128/jb.168.2.624-630.1986
复制
发表时间：
1986
影响因子：
3.2
通讯作者：
Taylor,BL
中科院分区：
文献类型：
--
作者：
Tribhuwan,RC;Johnson,MS;Taylor,BL
在大肠杆菌和鼠伤寒沙门氏菌中的趋化性研究中，没有再现cya和cpd突变体中磷酸转移酶趋化性的缺陷，这两种突变体先前被引用作为环GMP或环AMP中间体在信号转导中的证据。在cya突变体中，缺乏腺苷酸环化酶，另外环AMP是合成磷酸转移酶转运和趋化性所必需的蛋白质所必需的。然而，诱导细胞保留正常的磷酸转移酶趋化性后，环AMP被删除。S的cpd突变体中磷酸转移酶趋化性正常。具有升高的环GMP和环AMP水平的鼠伤寒沙门氏菌。S.鼠伤寒crr突变体缺乏酶III葡萄糖，其是葡萄糖转运系统的组分，并且是腺苷酸环化酶的调节剂。与环AMP预孵育后，crr突变体缺乏酶II葡萄糖介导的运输和趋化性，但其他趋化反应是正常的。它的结论是，环GMP不确定的频率翻滚，可能不是一个组件的转导途径。环AMP的唯一已知作用是合成某些受分解代谢物抑制的蛋白质。
Defects in phosphotransferase chemotaxis in cya and cpd mutants previously cited as evidence of a cyclic GMP or cyclic AMP intermediate in signal transduction were not reproduced in a study of chemotaxis in Escherichia coli and Salmonella typhimurium. In cya mutants, which lack adenylate cyclase, the addition of cyclic AMP was required for synthesis of proteins that were necessary for phosphotransferase transport and chemotaxis. However, the induced cells retained normal phosphotransferase chemotaxis after cyclic AMP was removed. Phosphotransferase chemotaxis was normal in a cpd mutant of S. typhimurium that has elevated levels of cyclic GMP and cyclic AMP. S. typhimurium crr mutants are deficient in enzyme III glucose, which is a component of the glucose transport system, and a regulator of adenylate cyclase. After preincubation with cyclic AMP, the crr mutants were deficient in enzyme II glucose-mediated transport and chemotaxis, but other chemotactic responses were normal. It is concluded that cyclic GMP does not determine the frequency of tumbling and is probably not a component of the transduction pathway. The only known role of cyclic AMP is in the synthesis of some proteins that are subject to catabolite repression.