Butyrate esterified to starch is released in the human gastrointestinal tract
Butyrate esterified to starch is released in the human gastrointestinal tract
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DOI：
10.3945/ajcn.111.017228
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发表时间：
2011-11-01
影响因子：
7.1
通讯作者：
Cobiac, Lynne
中科院分区：
文献类型：
--
作者：
Clarke, Julie M.;Topping, David L.;Cobiac, Lynne
背景：短链脂肪酸(SCFA)维持人类的结肠功能，可能有助于预防结肠疾病。一项与回肠造口师的研究表明，与特定的SCFAs酰化的淀粉大部分通过小肠存活，但在结肠中释放的百分比尚未确定。目的：目的是确定摄入的酯化丁酸盐在人体胃肠道中释放的百分比。设计：这项研究是一项随机、交叉、对照试验，包括基线和四个2周的周期，在此期间，16名志愿者每天食用低抗性淀粉和20和40克煮熟的高直链玉米淀粉(火腿：HAMS20或HAMS40)或丁基化火腿(HAMSB20或HAMSB40)。HAMSB20中含有31.8 mmoL酯化丁酸酯。结果：HAMSB40组大鼠粪便中游离丁酸浓度高于HAMSB20组(P<；0.005)和火腿组(P<；0.0001)，也高于基线数据(P<；0.0001)。粪便酯化丁酸浓度以HAMSB40(第1213天；P<；0.0001)组最高，HAMSB40(2-3天)和HAMSB20组高于HAMS组和基线水平(P<；0.0001)。HAMSB20和HAMSB40在胃肠道的释放量分别为26.8+/-1.0和50.2+/-2.4 mmol/d(12-13天)，分别占总摄入量的84.2+/-3.0%和79.0+/-3.1%。通过计算，摄入的酯化丁酸酯有57.2%在结肠中释放。微生物分析表明，这种释放可能主要是由Parabacteroiddisasonis促进的，随着HAMSB40(12-13天)的增加(P<；0.001)，HAMSB40的数量增加(P<；0.001)。结论：本研究表明，煮熟的丁酸酯淀粉有效地将酯化丁酸酯输送到人体结肠，具有改善人体肠道健康的潜力。该试验在澳大利亚临床试验注册中心注册为ACTRN012606000398505。AM J Clin Nutr 2011；94：1276-83。
Background: Short-chain fatty acids (SCFAs) maintain human colonic function and may help prevent colonic disease. A study with ileostomists showed that starches acylated with specific SCFAs largely survive passage through the small intestine, but the percentage released in the colon has not been established.Objective: The objective was to determine the percentage of ingested esterified butyrate released in the human gastrointestinal tract.Design: The study was a randomized, crossover, controlled trial consisting of baseline and four 2-wk periods during which 16 volunteers consumed diets low in resistant starch plus 20 and 40 g cooked high-amylose maize starch (HAMS: HAMS20 or HAMS40) or butyrylated HAMS (HAMSB20 or HAMSB40) daily. HAMSB20 contained 31.8 mmol esterified butyrate. Complete 48-h fecal collections were made on days 2-3 and 12-13 of each period.Results: Free fecal butyrate concentrations were higher after HAMSB40 than after HAMSB20 (P < 0.005) and HAMS (P < 0.0001) and higher than baseline data (P < 0.0001). Fecal esterified butyrate concentrations were highest in the HAMSB40 (days 1213; P < 0.0001) group, and concentrations in the HAMSB40 (days 2-3) and HAMSB20 groups were higher than those in the HAMS groups and those at baseline (P < 0.0001). Ingestion of HAMSB20 and HAMSB40 resulted in the release of 26.8 +/- 1.0 and 50.2 +/- 2.4 mmol butyrate/d (days 12-13) (84.2 +/- 3.0% and 79.0 +/- 3.1% of total ingested esterified butyrate), respectively, in the gastrointestinal tract. By calculation,; 57.2% of ingested esterified butyrate was released in the colon. Microbial analysis showed that this release was probably facilitated mainly by Parabacteroides distasonis, which increased in abundance with HAMSB40 (days 12-13) (P < 0.001).Conclusions: This study shows that cooked butyrylated starch delivers esterified butyrate to the human colon effectively and has the potential to improve human bowel health. This trial is registered in the Australian Clinical Trials Registry as ACTRN012606000398505. Am J Clin Nutr 2011;94:1276-83.