SC1/Hevin Identifies Early White Matter Injury After Ischemia and Intracerebral Hemorrhage in Young and Aged Rats
SC1/Hevin Identifies Early White Matter Injury After Ischemia and Intracerebral Hemorrhage in Young and Aged Rats
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DOI：
10.1097/nen.0b013e318256901c
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发表时间：
2012-06-01
影响因子：
3.2
通讯作者：
Schlichter, Lyanne C.
中科院分区：
文献类型：
--
作者：
Lively, Starlee;Schlichter, Lyanne C.
缺血性和出血性卒中后白色损害的进展可加重初始损伤，但对所涉及的过程知之甚少。我们发现，抗粘附的基质细胞糖蛋白SC 1是一种新的早期标记物白色物质损伤的3种模型急性损伤大鼠纹状体：短暂性局灶性缺血，脑出血，针刺伤口。SC1仅限于受损部分的轴突束，毗邻中风病变的成年和老年大鼠。SC1在脑出血后第1、3天和缺血后第7天达到高峰。SC1阳性束通常表达降解的髓鞘碱性蛋白和淀粉样前体蛋白，这是轴突损伤的标志物。在血肿边缘，SC1可见于少数轴突束，保留髓鞘碱性蛋白染色。在这些束中，点状SC1染色填充单个轴突，延伸超出泛轴突神经丝和NF 200的核心，并且当存在时，在髓磷脂碱性蛋白染色内部或与髓磷脂碱性蛋白染色重叠。老年大鼠在两种类型的中风后SC1（和淀粉样前体蛋白）较少，表明轴突反应减少。SC 1还标记淀粉样前体蛋白阳性轴突束沿着针穿刺道的盐水注射大鼠，因此，SC 1似乎表征受损的纹状体白色物质损伤后，多种类型的损伤。
The progression of white matter damage after ischemic and hemorrhagic strokes can exacerbate the initial injury, but little is known about the processes involved. We show that the antiadhesive matricellular glycoprotein SC1 is a novel early marker of white matter damage in 3 models of acute injury in the rat striatum: transient focal ischemia, intracerebral hemorrhage, and a needle penetration wound. SC1 was restricted to the damaged portions of axon bundles that bordered stroke lesions in young-adult and aged rats. SC1 peaked at 1 and 3 days after intracerebral hemorrhage and at 7 days after ischemia. The SC1-positive bundles usually expressed degraded myelin basic protein and amyloid precursor protein, a marker of axonal injury. At the hematoma edge, SC1 was seen in a few axon bundles that retained myelin basic protein staining. In these bundles, punctate SC1 staining filled individual axons, extended beyond a core of pan-axonal neurofilament and NF200 and was inside or overlapped with myelin basic protein staining when it was present. Aged rats had less SC1 (and amyloid precursor protein) after both types of stroke, suggesting a reduced axonal response. SC1 also labeled amyloid precursor protein-positive axon bundles along the needle penetration tract of saline-injected rats; thus, SC1 appears to characterize damaged striatal white matter damage after multiple types of injury.