A tandem chromatographic column method for assaying cAMP-dependent protein kinase and protein kinase C with synthetic peptide substrates.
A tandem chromatographic column method for assaying cAMP-dependent protein kinase and protein kinase C with synthetic peptide substrates.
复制标题
用于使用合成肽底物测定 cAMP 依赖性蛋白激酶和蛋白激酶 C 的串联色谱柱方法。
DOI：
10.1016/0003-2697(88)90583-0
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发表时间：
1988
影响因子：
2.9
通讯作者：
Londos,C
中科院分区：
文献类型：
--
作者：
Egan,JJ;Chang,MK;Londos,C
设计了一种检测蛋白激酶的方法，它可以磷酸化蛋白激酶，如cAMP依赖的蛋白激酶，或糖原合成酶肽，它是蛋白激酶C的优良底物。通过串联的阳离子交换树脂和阴离子交换树脂对反应混合物进行顺序处理后，背景样品中的放射性几乎为零，磷酸化多肽的产率很高。这种方法减少了人工、放射性、酶的需求，并降低了检测蛋白激酶的成本。
A method was devised for assaying protein kinases that phosphorylate either Kemptide, such as cAMP-dependent protein kinase, or a glycogen synthase peptide, which is an excellent substrate for protein kinase C. Upon sequential processing of reaction mixtures through tandem columns of cation and anion exchange resins, radioactivity in background samples is nearly nil and the yield of phosphorylated peptides is high. This method reduces labor, radioactivity, enzyme requirements, and costs of assaying protein kinases.