Differential impact of pavlovian drug conditioned stimuli on in vivo dopamine transmission in the rat accumbens shell and core and in the prefrontal cortex
Differential impact of pavlovian drug conditioned stimuli on in vivo dopamine transmission in the rat accumbens shell and core and in the prefrontal cortex
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DOI：
10.1007/s00213-006-0560-7
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发表时间：
2007-04-01
影响因子：
3.4
通讯作者：
Di Chiara, Gaetano
中科院分区：
文献类型：
--
作者：
Bassareo, Valentina;De Luca, Maria Antonietta;Di Chiara, Gaetano
理条件刺激（CSs）的巴甫洛夫协会与加强药物（US）被认为在收购，维护和复发的药物dependency.Objective目的本研究的目的是探讨微透析巴甫洛夫药物CSs对行为的影响和对基础和药物刺激的多巴胺（DA）在三个终端DA领域：核壳，核心和前额叶皮层（PFCX）。方法采用Fonzies填充箱（FFB，CS）训练大鼠10 min，然后分别给予吗啡（1 mg/kg）、尼古丁（0.4mg/kg）和生理盐水，每天1次，连续3d。给药或生理盐水后10 h，给假条件化大鼠提供FFB。将微透析探针植入大鼠的壳、芯和PFCX中。本文研究了吗啡和尼古丁条件刺激对多巴胺（DA）的影响及对药物反应的影响。结果药物CSs能引起兴奋反应，并在壳和PFCX中释放DA，但在核中不释放。预先暴露于吗啡CS增强DA释放吗啡的挑战，在壳，但不是在核心和PFCX。这种效果与吗啡的挑战剂量有关，并且是刺激特异性的，因为食物CS不增强对吗啡的壳DA反应。结论药物CS可刺激海马壳层和内侧PFCX的DA释放，并特异性地增强初级兴奋药物对DA传递的作用。
Rationale Conditioned stimuli (CSs) by pavlovian association with reinforcing drugs (US) are thought to play an important role in the acquisition, maintenance and relapse of drug dependence.Objective The aim of this study was to investigate by microdialysis the impact of pavlovian drug CSs on behaviour and on basal and drug-stimulated dopamine (DA) in three terminal DA areas: nucleus accumbens shell, core and prefrontal cortex (PFCX). Methods Conditioned rats were trained once a day for 3 days by presentation of Fonzies filled box (FFB, CS) for 10 min followed by administration of morphine (1 mg/kg), nicotine (0.4 mg/kg) or saline, respectively. Pseudo-conditioned rats were presented with the FFB 10 h after drug or saline administration. Rats were implanted with microdialysis probes in the shell, core and PFCX. The effect of stimuli conditioned with morphine and nicotine on DA and on DA response to drugs was studied. Results Drug CSs elicited incentive reactions and released DA in the shell and PFCX but not in the core. Pre-exposure to morphine CS potentiated DA release to morphine challenge in the shell but not in the core and PFCX. This effect was related to the challenge dose of morphine and was stimulus-specific since a food CS did not potentiate the shell DA response to morphine. Pre-exposure to nicotine CS potentiated DA release in the shell and PFCX.Conclusion The results show that drug CSs stimulate DA release in the shell and medial PFCX and specifically potentiate the primary stimulant drug effects on DA transmission.