Morphology and molecular phylogeny of the marine gregarine parasite Selenidium oshoroense n. sp. (Gregarina, Apicomplexa) isolated from a Northwest Pacific Hydroides ezoensis Okuda 1934 (Serpulidae, Polychaeta)
Morphology and molecular phylogeny of the marine gregarine parasite Selenidium oshoroense n. sp. (Gregarina, Apicomplexa) isolated from a Northwest Pacific Hydroides ezoensis Okuda 1934 (Serpulidae, Polychaeta)
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海洋gregarine寄生虫Selenidium oshoroense n的形态学和分子系统发育。
DOI：
10.1007/s12526-017-0643-1
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发表时间：
2018
影响因子：
1.6
通讯作者：
T Horiguchi
中科院分区：
文献类型：
--
作者：
KC Wakeman;T Horiguchi
在这项研究中，我们描述了一种新的海洋绿藻寄生虫，硒。sp.，从hydroides ezoensisOkuda 1934 （Serpulidae, Polychaeta）中分离得到。滋养体（摄食阶段）oshoroensen。sp.从h。采集自日本北海道奥肖罗潮间带海岸的潮藻，利用扫描电镜（SEM）和透射电镜（TEM）进行分析，并利用18S rDNA进行分子系统发育分析。营养体ofS。oshoroensen。sp平均长120 μm，宽13 μm。扫描电镜和透射电镜观察发现，细胞表面有23条纵向排列的表胞褶皱。花序梗、多膜结合的螺旋和包涵体也被观察到，表明了表面介导的营养。在透射电镜下观察的宿主肠道组织切片也显示了细胞顶端的myzocytic摄食，这表明myzocytic可能是来自管状多毛体的硒细胞分支的共同特征。18S rDNA类群的分子系统发育分析。oshoroensen。属从管状多毛体中硒的一个分支，姐妹toS。serpulae。这两个18S rDNA序列分别来源于s。oshoroensen。sp.的相似度为0.997，相似toS值分别为0.965和0.966。serpulae。美国oshoroensen。sp.是从S.分化而来。浆膜，基于胞外褶皱表面没有横向条纹和生态位（寄主）的差异。类群的系统发育分析支持形态学差异。oshoroensen。sp.分离株，排除s。serpulae。
In this study, we describe a novel marine gregarine parasite,Selenidium oshoroensen. sp., isolated fromHydroides ezoensisOkuda 1934 (Serpulidae, Polychaeta). Trophozoites (feeding stages) ofS. oshoroensen. sp. were isolated from the gut ofH. ezoensiscollected from the intertidal shore near Oshoro, Hokkaido, Japan, and prepared for analysis with scanning and transmission electron microscopy (SEM and TEM), and molecular phylogenetic analysis using 18S rDNA. Trophozoites ofS. oshoroensen. sp. were, on average, 120 μm long and 13 μm wide. Observation of the cells under SEM and TEM revealed 23 longitudinally running epicytic folds on the surface of the cells. Peduncles, multimembrane-bound whorls, and inclusions were also observed and were indicative of surface-mediated nutrition. Myzocytotic feeding at the apical end of the cell was also indicated in sections of host gut tissue that were viewed under TEM, suggesting that myzocytosis could be a shared feature among this clade ofSelenidiumfrom tube-forming polychaetes. Molecular phylogenetic analysis of the 18S rDNA groupedS. oshoroensen. sp. within a clade ofSelenidiumfrom tube-forming polychaetes, sister toS. serpulae. The two 18S rDNA sequences generated from separate isolates ofS. oshoroensen. sp. had a similarity of 0.997, but were 0.965 and 0.966 similar toS. serpulae. S. oshoroensen. sp. was differentiated from,S. serpulae, based on the absence of transverse striations on the surface of the epicytic folds and difference in ecological niche (host). Morphological differences were supported in phylogenetic analysis which groupedS. oshoroensen. sp. isolates, to the exclusion ofS. serpulae.
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微管和Archigregarine、Selenidium Fallax 的弯曲波传播。
影响因子：
2.8
作者：
J. Mellor;H. Stebbings
通讯作者：
H. Stebbings
Gregarinia（孢子虫）的系统发育
DOI：
10.1007/bf00927181
发表时间：
1984
期刊：
Origins of Life
影响因子：
--
作者：
J. Théodoridès
通讯作者：
J. Théodoridès
影响因子：
2.5
作者：
Schrevel, Joseph;Valigurova, Andrea;Guillou, Laure
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Guillou, Laure
太平洋Archigregarines (Apicomplexa) 的分子系统发育，包括Veloxyium leptosynaptae n. 的描述。
影响因子：
2.2
作者：
K. Wakeman;B. Leander
通讯作者：
B. Leander
影响因子：
2.5
作者：
Leander, Brian S.
通讯作者：
Leander, Brian S.