Micro/Nanometer-Structured Scaffolds for Regeneration of Both Cartilage and Subchondral Bone
Micro/Nanometer-Structured Scaffolds for Regeneration of Both Cartilage and Subchondral Bone
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DOI：
10.1002/adfm.201806068
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发表时间：
2019-01-24
影响因子：
19
通讯作者：
Wu, Chengtie
中科院分区：
文献类型：
--
作者：
Deng, Cuijun;Lin, Rongcai;Wu, Chengtie
由于软骨和软骨下骨具有明显不同的生理特性，骨软骨缺损的治疗在临床实践中仍然是一个巨大的挑战。在这项研究中，可控的表面微/纳米结构的生物活性支架在生物材料化学的组合是利用来解决这个问题。通过将3D打印与水热工艺相结合，成功地制备了具有可控表面微/纳米结构的生物活性生物材料模型，即硼氢化钙（BRT）支架。结果表明，微/纳米磷酸钙晶体在BRT支架表面的生长，通过修复支架表面的微裂纹，显著提高了支架的抗压强度。微/纳米结构表面通过激活整合素α vb 1和α 5 b1异二聚体明显促进软骨细胞的扩散和分化，调节细胞形态，并通过整合素α 5 b1和RhoA的协同作用促进兔骨髓基质细胞（rBMSCs）的成骨分化，其中微棒表面对软骨细胞和rBMSCs的分化表现出最高的刺激作用。体内研究表明，3D打印支架的微/纳米结构表面明显促进软骨和软骨下骨组织的再生。这项研究表明，在多孔3D支架中构建可控的微/纳米结构表面提供了一种智能策略，以诱导骨软骨再生的双系生物活性。
Treatment of osteochondral defects remains a great challenge in clinical practice because cartilage and subchondral bone possess significantly different physiological properties. In this study, the controlled surface micro/nanometer structure of bioactive scaffolds in a combination of biomaterial chemistry is harnessed to address this issue. Model bioactive biomaterials, bredigite (BRT) scaffolds, with controlled surface micro/nanostructure are successfully fabricated by combining 3D printing with a hydrothermal process. It is found that the growth of micro/nano-calcium phosphate crystals on the surface of BRT scaffolds notably enhances their compressive strength by healing the microcracks on the strut surface. The micro/nanostructured surface distinctly facilitates the spread and differentiation of chondrocytes by activating integrin alpha vb1 and alpha 5b1 heterodimers, regulates cell morphology, and promotes osteogenic differentiation of rabbit bone marrow stromal cells (rBMSCs) through the synergetic effect of integrin alpha 5b1 and RhoA, in which the microrod surface demonstrates the highest stimulatory effect on the differentiation of chondrocytes and rBMSCs. The in vivo study shows that the micro/nanostructured surface of the 3D printed scaffolds obviously promotes the regeneration of both cartilage and subchondral bone tissues. This study suggests that the construction of controlled micro/nanostructured surface in porous 3D scaffolds offers a smart strategy to induce bilineage bioactivities for osteochondral regeneration.