Different peripheral tissue injury induces differential phenotypic changes of spinal activated microglia.
Different peripheral tissue injury induces differential phenotypic changes of spinal activated microglia.
复制标题
不同周围组织损伤引起脊髓激活小胶质细胞的差异表型变化
DOI：
10.1155/2013/901420
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发表时间：
2013
影响因子：
--
通讯作者：
Fu KY
中科院分区：
文献类型：
--
作者：
Li K;Tan YH;Light AR;Fu KY
本研究的目的是探讨不同疼痛模型中与脊髓小胶质细胞激活相关的细胞标志物表达的差异。采用免疫组织化学和Western blotting方法，定量比较了完全弗氏佐剂(CFA)注射、福尔马林注射和慢性压迫性损伤(CCI)3种疼痛动物模型脊髓中CD45、CD68和MHC-I类抗原以及CD11b和Iba-1的表达。结果表明，CCI模型和福尔马林模型脊髓小胶质细胞CD45和MHC-I类抗原表达明显上调，CD11b和Iba-1染色增强，而CFA诱导的炎性痛模型未见明显的形态学改变。CD68仅在CCI模型中表达。我们的发现提示，不同的外周组织损伤会产生与脊髓小胶质细胞激活相关的不同表型变化；周围神经损伤可能诱导脊髓小胶质细胞获得这些免疫分子表型变化。
The purpose of this study is to investigate the possible different cellular marker expression associated with spinal cord microglial activation in different pain models. Immunohistochemistry and western blotting analysis of CD45, CD68, and MHC class I antigen as well as CD11b and Iba-1 in the spinal cord were quantitatively compared among widely used three pain animal models, complete Freund's adjuvant (CFA) injection, formalin injection, and chronic constriction injury (CCI) models. The results showed that significant upregulated expressions of CD45 and MHC class I antigen in spinal microglia as well as morphological changes with increased staining with CD11b and Iba-1 were seen in CCI and formalin models and not found in CFA-induced inflammatory pain model. CD68 expression was only detected in CCI model. Our findings suggested that different peripheral tissue injuries produced differential phenotypic changes associated with spinal microglial activation; peripheral nerve injury might induce spinal microglia to acquire these immunomolecular phenotypic changes.
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影响因子：
2.8
作者：
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通讯作者：
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