MOLECULAR-CLONING OF A CD28 CDNA BY A HIGH-EFFICIENCY COS CELL EXPRESSION SYSTEM
MOLECULAR-CLONING OF A CD28 CDNA BY A HIGH-EFFICIENCY COS CELL EXPRESSION SYSTEM
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DOI：
10.1073/pnas.84.23.8573
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发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
SEED, B
中科院分区：
文献类型：
--
作者：
ARUFFO, A;SEED, B
CD28(Tp44)是一种人类T细胞特异性同源二聚体表面蛋白，可能参与T细胞的活化。通过在COS细胞中瞬时表达的一种简单高效的克隆策略，我们获得了一个编码CD28的cDNA克隆。这一策略的核心是使用一种有效的方法来制备大的质粒库。这些文库被引入到COS细胞中，其中表面抗原的瞬时表达允许通过单抗结合的方式分离cDNA。CD28基因编码一种高度糖基化的膜蛋白，与免疫球蛋白超家族具有同源性，并指导转基因COS细胞产生同源二聚体。
CD28 (Tp44) is a human T-cell-specific homodimer surface protein that may participate in T-cell activation. We have isolated a cDNA clone encoding CD28 by a simple and highly efficient cloning strategy based on transient expression in COS cells. Central to this strategy is the use of an efficient method to prepare large plasmid cDNA libraries. The libraries are introduced into COS cells, where transient expression of surface antigen allows the isolation of cDNAs by way of monoclonal antibody binding. The CD28 cDNA encodes a highly glycosylated membrane protein with homology to the immunoglobulin superfamily and directs the production of a homodimer in transfected COS cells.