Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations
Establishment of a novel human acute myeloblastic leukemia cell line (YNH-1) with t(16;21), t(1;16) and 12q13 translocations
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DOI：
10.1038/sj.leu.2400594
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发表时间：
1997-04
期刊：
影响因子：
11.4
通讯作者：
K. Yamamoto;H. Hamaguchi;K. Nagata;M. Kobayashi;F. Tanimoto;M. Taniwaki
中科院分区：
文献类型：
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作者：
K. Yamamoto;H. Hamaguchi;K. Nagata;M. Kobayashi;F. Tanimoto;M. Taniwaki
t（16; 21）（p11; q22）易位是在几种类型的人类急性髓细胞白血病（AML）中观察到的非随机染色体畸变，而der（16）t（1; 16）和12 q13处的染色体重排经常在实体瘤中发现。从一名46岁男性AML（M1）患者的外周血细胞中建立了一种新的细胞系YNH-1，该患者携带t（16; 21）和t（1; 16）易位。作为粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-3（IL-3）依赖性细胞系，YNH-1的倍增时间为82 h，持续时间超过20个月。形态学上，YNH-1细胞是自由漂浮的未成熟成髓细胞，细胞核分叶状，胞浆中有空泡。髓过氧化物酶染色阳性，α-萘丁酸酯酶和氯乙酸酯酶染色阴性。在表面标记分析中，YNH-1细胞对CD 13、CD 33和CD 34呈阳性。染色体分析显示46，XY，der（16）t（16; 21）（p11; q22）t（1; 16）（q12; q13），der（21）t（16; 21）（p11; q22），der（6）t（6; 12）（q13; q13），der（12）t（6; 12）（q21; q13）。这些易位证实了荧光原位杂交（FISH）研究与ERG-YAC克隆和染色体特异性DNA文库。FUS/ERG和ERG/FUS嵌合体转录本均通过逆转录-聚合酶链反应（RT-PCR）分析鉴定。因此，YNH-1可能是一个有用的工具，阐明AML的病理生理和分子机制与t（16; 21），t（1; 16）和12 q13易位。
The t (16; 21)(p11; q22) translocation is a non-random chromosomal aberration observed in several types of human acute myeloblastic leukemia (AML), whereas the der (16) t (1; 16) and chromosome rearrangements at 12q13 are frequently found in solid tumors. A novel cell line YNH-1 was established from peripheral blood cells of a 46-year-old male with AML (M1) carrying t (16; 21) and t (1; 16) translocations. YNH-1 has been maintained with a doubling time of 82 h for more than 20 months as a granulocyte colony-stimulating factor (G-CSF), granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) dependent line. Morphologically YNH-1 cells were free-floating immature myeloblasts with lobulated nuclei and vacuoles in the cytoplasm. They were positive for myeloperoxidase but negative for α-naphthyl butylate esterase and chloroacetate esterase stainings. In surface marker analysis YNH-1 cells were positive for CD13, CD33 and CD34. Chromosomal analysis showed 46, XY, der (16) t (16; 21)(p11; q22) t (1; 16)(q12; q13), der (21) t (16; 21)(p11; q22), der (6) t (6; 12)(q13; q13), der (12) t (6; 12)(q21; q13). These translocations were confirmed by fluorescence in situ hybridization (FISH) studies with the ERG-YAC clone and chromosome-specific DNA libraries. Both the FUS/ERG and ERG/FUS chimeric transcripts were identified by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Thus, YNH-1 could be a useful tool for elucidating the pathophysiology and molecular mechanism in AML with t (16; 21), t (1; 16) and 12q13 translocations.