Conformations of co-translational folding intermediates
Conformations of co-translational folding intermediates
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DOI：
10.2174/0929866053005908
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发表时间：
2005-02-01
影响因子：
1.6
通讯作者：
Clark, PL
中科院分区：
文献类型：
--
作者：
Evans, MS;Clark, TF;Clark, PL
虽然体外实验对我们对蛋白质折叠的理解做出了很大贡献，但我们对蛋白质在更复杂的细胞环境中如何折叠的了解却少得多。这篇综述总结了我们目前对最早的体内折叠中间体的了解：核糖体合成过程中新生多肽所采用的构象。还讨论了与细胞环境中成功折叠相关的挑战，包括路径外聚集和大分子拥挤。
While in vitro experiments have contributed much to our understanding of protein folding, we know much less about how proteins fold in the more complex environment of the cell. This review summarizes our current knowledge of the earliest in vivo folding intermediates: the conformations adopted by nascent polypeptides during synthesis by the ribosome. The challenges related to successful folding in the cellular environment, including off-pathway aggregation and macromolecular crowding, are also discussed.