Implications of blood contamination for assessment of local cellular immunity in the endocervix
Implications of blood contamination for assessment of local cellular immunity in the endocervix
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DOI：
10.1089/088922204323087796
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发表时间：
2004-05-01
影响因子：
1.5
通讯作者：
Duerr, A
中科院分区：
文献类型：
--
作者：
Quayle, AJ;Shah, M;Duerr, A
宫颈细胞刷提供了一种工具，用于对宫颈内T细胞进行采样，以评估局部免疫力。然而，大多数以前的研究中，艾滋病毒血清阳性的妇女排除了含有血液的样本，因此分析了选定的患者群体。通过对来自两个连续细胞刷和同时收集的血液样本的T淋巴细胞进行多参数流式细胞术分析，本研究发现血液污染对正常女性宫颈T细胞表型参数的影响极小。宫颈内样本中血液的后果最终取决于每项研究的设计和目的，但这些数据表明研究可以更具包容性，不应自动丢弃含有红细胞的样本。
Cervical cytobrushes provide a tool to sample endocervical T cells for assessment of local immunity. However, most previous studies in HIV-seropositive women have excluded samples containing blood and hence have analyzed selected populations of patients. As determined by multiple-parameter flow cytometric analysis of T lymphocytes from two sequential cytobrushes and concurrently collected blood samples, this study found a minimal effect of blood contamination on cervical T cell phenotypic parameters in normal women. The consequences of blood in endocervical samples will ultimately depend on the design and objective of each study, but these data suggest studies could be more inclusive and should not automatically discard samples that contain red blood cells.