Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells.
Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells.
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小檗碱对LPS诱导的猪肠上皮细胞NF-κB/MAPK信号通路及相关炎症细胞因子表达的影响
DOI：
10.1177/1753425920930074
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发表时间：
2020-10
期刊：
影响因子：
3.2
通讯作者：
Zili W
中科院分区：
文献类型：
--
作者：
Zhu Z;Xueying L;Chunlin L;Wen X;Rongrong Z;Jing H;Meilan J;Yuwei X;Zili W
小檗碱是一种从黄连、黄柏等药用植物中提取的生物碱。它具有抗炎、抗肿瘤和抗氧化的特性，并调节血糖和脂质代谢。本研究探讨了小檗碱对LPS诱导的猪肠上皮细胞（IPEC-J2）屏障功能和炎症损伤的保护作用机制。我们首先评估了小檗碱和LPS对细胞活力的影响。用5 μg/ml LPS处理IPEC-J2细胞1 h建立炎症模型，再用75、150、250 μg/ml小檗碱进行进一步实验。RT-PCR法检测IL-1β、IL-6、TNF-α的表达。Western blot检测NF-κB/MAPK信号通路关键蛋白（i -κB α、p -κB α、p65、p-p65、c-Jun n -末端激酶（JNK）、p-JNK、p38、p-p38、ERK1/2、p-ERK1/2）。暴露于LPS后，小鼠IL-1β、IL-6、TNF-α mRNA水平和p- κ b α p-p65蛋白水平显著升高。小檗碱预处理可降低炎症因子的表达，并与其浓度呈正相关，剂量依赖性地抑制i - κ b α、p- κ b α、p-p65、p-p38和JNK的表达。上述结果表明，用小檗碱预处理肠上皮细胞可有效预防和治疗大肠杆菌引起的断奶仔猪腹泻。
Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that pre-treating intestinal epithelial cells with berberine was useful in preventing and treating diarrhoea induced by Escherichia coli in weaned pigs.
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影响因子：
5.5
作者：
Egan, LJ;de Lecea, A;Kagnoff, MF
通讯作者：
Kagnoff, MF
影响因子：
3.1
作者：
BARTON, BE;JACKSON, JV
通讯作者：
JACKSON, JV
影响因子：
11.2
作者：
Shi, Lin;Fang, Biao;Ju, Xianghong
通讯作者：
Ju, Xianghong
DOI：
10.1152/ajpgi.00515.2004
发表时间：
2005-07-01
影响因子：
4.5
作者：
Sun, J;Hobert, ME;Madara, JL
通讯作者：
Madara, JL
影响因子：
4.4
作者：
Gioannini, Theresa L.;Weiss, Jerrold P.
通讯作者：
Weiss, Jerrold P.