How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study
How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study
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DOI：
10.1007/s10658-008-9271-8
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发表时间：
2008-07-01
影响因子：
1.8
通讯作者：
Rung, Jesper
中科院分区：
文献类型：
--
作者：
Collinge, David B.;Jensen, Michael K.;Rung, Jesper
“功能基因组学”的概念是指用于基因组功能表征的方法。所使用的方法为研究植物防御机制的性质和作用提供了新的机会。与拟南芥、白杨和水稻不同，大麦的全基因组序列是不可用的。在这种情况下，大麦基因表达数据的分析起着关键作用，获得洞察到个别基因产物的功能特性。许多基因在受到病原体攻击后被转录激活，这些基因通常有助于构成抗病性基础的防御机制。利用大规模的互补DNA文库的建设和全基因组的转录本配置文件的植物暴露于生物胁迫提供了所需的数据，以推动有关新发现的基因的功能的假设。在本文中，我们说明了如何公开可用的基因表达数据已被证明是有效的植物防御反应的研究，使一个具有成本效益的工作流程，从分离的基因转录本生物胁迫后的生物功能的阐明。
The concept 'functional genomics' refers to the methods used for the functional characterisation of genomes. The methods utilised provide new opportunities for studying the nature and role of defence mechanisms in plants. Unlike Arabidopsis, poplar and rice, the full genomic sequence of barley is not available. In this case, the analysis of barley gene expression data plays a pivotal role for obtaining insight into the functional characterisation of individual gene products. Many genes are activated transcriptionally following attack by pathogens and these often contribute to the defence mechanisms which underlie disease resistance. The use of large-scale complementary DNA library constructions and genome-wide transcript profiles of plants exposed to biotic stress provide the data required to drive hypotheses concerning the function of newly identified genes. In this paper, we illustrate how publicly available gene expression data has proved valid for studies of plant defence responses; enabling a cost-effective workflow starting from isolated gene transcripts to elucidation of biological function upon biotic stress.