Gene expression patterns of invertase gene families and modulation of the inhibitor gene in tomato sucrose metabolism.
Gene expression patterns of invertase gene families and modulation of the inhibitor gene in tomato sucrose metabolism.
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DOI：
10.4238/2013.january.24.1
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发表时间：
2013-01
期刊：
影响因子：
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通讯作者：
Y. Zhang;A. Zhang;J. Jiang
中科院分区：
文献类型：
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作者：
Y. Zhang;A. Zhang;J. Jiang
番茄中不同类型蔗糖代谢的基因表达模式是高度可变和可遗传的。这种遗传变异会导致相当大的功能差异。我们研究了转化酶(Inv)基因家族和转化酶抑制基因(INH)在番茄伸长根、下胚轴和果实中的表达模式。Micro-Tom和L.chmielewskii)。我们发现Lin6基因在营养生长阶段起着重要的作用。Lin5和Lin7在Micro-Tom中不表达，但在L.chmielewskii中表达。在生殖生长阶段，蔗糖Inv基因家族的总体相对表达水平显著低于Micro-Tom，低达数百倍。该基因在切氏乳杆菌的隔膜中不表达。我们认为，番茄果实蔗糖积累的差异主要是由于果实生长后期转化酶基因家族表达的差异所致。
Patterns of gene expression in the different types of sucrose metabolism in the tomato are highly variable and heritable. This genetic variation causes considerable functional differences. We examined the patterns of expression of invertase (Inv) gene families and an invertase inhibitor (INH) gene involved in elongating roots, hypocotyls, and fruit of the tomato (Lycopersicon esculentum cv. Micro-Tom and L. chmielewskii) through a real-time quantitative PCR analysis. We found that the Lin6 gene plays an important role in the vegetative growth stage. Lin5 and Lin7 did not express in Micro-Tom, but did express in L. chmielewskii. Overall relative expression levels of sucrose Inv gene families were significantly lower in L. chmielewskii during the reproductive growth stage than in Micro-Tom, being up to hundreds of times lower. It was not expressed in the dissepiment in L. chmielewskii. We suggest that differences in sucrose accumulation in tomato fruit is mainly due to differentially expressed invertase gene families at the later fruit growth stages.