Relationships between nucleic acid levels and egg production rates in Acartia bifilosa:: implications for growth assessment of copepods in situ
Relationships between nucleic acid levels and egg production rates in Acartia bifilosa:: implications for growth assessment of copepods in situ
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DOI：
10.3354/meps262163
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发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
Gorokhova, E
中科院分区：
文献类型：
--
作者：
Gorokhova, E
为了评估基于RNA的指标在桡足动物生长评估中的适用性，我们根据产蛋实验估算的生长速率校准了双歧软足动物（Acartia bifilosa）的核酸浓度，并检验了RNA、DNA水平以及RNA:DNA比值与生长速率之间的关系。此外，为了研究温度和食物供应对体重特异性繁殖力与核酸水平之间关系的影响，在9℃和16℃的条件下，分别在3种食物浓度下进行孵育。核酸浓度与其比值和体重产蛋率呈正相关。总的来说，RNA浓度是特定生长率的最佳预测因子。测量的变量和女性体型之间没有相关性。当通过控制摄食方式提高产蛋量时，RNA浓度和RNA:DNA比值同时增加。生长和RNA指标均不受温度的显著影响，而在高食物水平和低温条件下，DNA浓度显著增加。rna -生长关系中缺乏温度依赖性，这使得它们可以直接应用于波罗的海北部地区双歧螺旋藻夏季种群的原位生长估计。
To evaluate the applicability of RNA-based indices in copepod growth assessment, concentrations of nucleic acids in Acartia bifilosa were calibrated against growth rates estimated via egg production experiments, and the relationships between levels of RNA, DNA, and the RNA:DNA ratio and growth rates were examined. Furthermore, to investigate effects of temperature and food availability on the relationships between weight-specific fecundity and nucleic acid levels, incubations were carried out at 9 and 16degreesC, each at 3 food concentrations. There were positive relationships between nucleic acid concentrations and their ratios and weight-specific egg production rates. Overall, RNA concentration was the best predictor of specific growth rate. No correlations between either of the measured variables and female body size were observed. When egg production was elevated by manipulating the feeding regime, RNA concentration and the RNA:DNA ratio increased in concert. Neither growth nor RNA indices were significantly affected by temperature, while a significant increase in DNA concentrations was observed at high food levels and low temperatures. The lack of temperature dependence in RNA-growth relationships allows their direct application for in situ growth estimates in summer populations of A. bifilosa in the northern Baltic Proper.