Molecular cloning and expression of gene fragments from corynebacteriophage beta encoding enzymatically active peptides of diphtheria toxin
Molecular cloning and expression of gene fragments from corynebacteriophage beta encoding enzymatically active peptides of diphtheria toxin
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编码白喉毒素酶活性肽的β棒状噬菌体基因片段的分子克隆和表达
DOI：
10.1128/jb.156.2.680-685.1983
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发表时间：
1983
影响因子：
3.2
通讯作者：
Robert J Collier
中科院分区：
文献类型：
--
作者：
Rodney K. Tweten;Robert J Collier
从杆状噬菌体β - vir tox+中分离出两个编码白喉毒素片段A和链终止片段CRM45的限制性内切片段，克隆到大肠杆菌pBR322质粒中。含有重组质粒的克隆产生的基因产物在催化延伸因子2的ADP核糖基化中具有活性，并且与白喉毒素抗血清有反应。毒素相关肽主要存在于质周室中，在合成后1 - 2小时内降解为无免疫反应性的形式。这两个基因片段的表达似乎都起源于白喉毒素启动子。
Two restriction fragments from corynebacteriophage beta vir tox+ that encode peptides similar to diphtheria toxin fragment A and the chain termination fragment, CRM45, have been cloned into Escherichia coli in plasmid pBR322. Clones containing the recombinant plasmids produced gene products that were active in catalyzing the ADP ribosylation of elongation factor 2 and were reactive with diphtheria toxin antiserum. Toxin-related peptides were found primarily in the periplasmic compartment and were degraded to nonimmunoreactive forms within 1 to 2 h of synthesis. The expression of both gene fragments appears to have originated from the diphtheria toxin promoter.
抗体导向的细胞毒剂：使用单克隆抗体引导毒素A链作用于结直肠癌细胞。
DOI：
10.1073/pnas.77.8.4539
发表时间：
1980
影响因子：
11.1
作者：
Gilliland,DG;Steplewski,Z;Collier,RJ;Mitchell,KF;Chang,TH;Koprowski,H
通讯作者：
Koprowski,H
DOI：
10.1073/pnas.78.8.4950
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KAGAN, BL;FINKELSTEIN, A;COLOMBINI, M
通讯作者：
COLOMBINI, M