Modulation of mitogenic activity of fibroblast growth factors by inorganic polyphosphate
Modulation of mitogenic activity of fibroblast growth factors by inorganic polyphosphate
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DOI：
10.1074/jbc.m303468200
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发表时间：
2003-07-18
影响因子：
4.8
通讯作者：
Ohshiro, M
中科院分区：
文献类型：
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作者：
Shiba, T;Nishimura, D;Ohshiro, M
在培养基中添加无机聚磷酸盐（poly(P)）可促进正常人成纤维细胞的增殖。聚P还能增强酸性成纤维细胞生长因子（FGF-1）和碱性成纤维细胞生长因子（FGF-2）的有丝分裂活性。观察到poly(P)和FGF-2之间的物理相互作用，并且poly(P)在物理和功能上都稳定了FGF-2。此外，聚(P)促进了FGF-2与其细胞表面受体的结合。由于poly(P)广泛分布于哺乳动物组织中，它可能是一种自发的fgf调节剂。
The proliferation of normal human fibroblast cells was enhanced by the addition of inorganic polyphosphate ( poly( P)) into culture media. The mitogenic activities of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) were also enhanced by poly( P). A physical interaction between poly( P) and FGF-2 was observed, and FGF-2 was both physically and functionally stabilized by poly( P). Furthermore, poly( P) facilitated the FGF-2 binding to its cell surface receptors. Because poly( P) is widely distributed in mammalian tissues, it may be a spontaneous modulator of FGFs.