MULTIPLE EXTRACELLULAR SIGNALS GOVERN THE PRODUCTION OF A MORPHOGENETIC PROTEIN INVOLVED IN AERIAL MYCELIUM FORMATION BY STREPTOMYCES-COELICOLOR
MULTIPLE EXTRACELLULAR SIGNALS GOVERN THE PRODUCTION OF A MORPHOGENETIC PROTEIN INVOLVED IN AERIAL MYCELIUM FORMATION BY STREPTOMYCES-COELICOLOR
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DOI：
10.1101/gad.7.5.895
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发表时间：
1993-05-01
影响因子：
10.5
通讯作者：
LOSICK, R
中科院分区：
文献类型：
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作者：
WILLEY, J;SCHWEDOCK, J;LOSICK, R
丝状细菌天蓝色链霉菌气生菌丝体的形成部分取决于一种叫做SapB的小的形态发生蛋白。一个代表性的秃头（bld）突变体，这是在气生菌丝体形成受阻，都有缺陷的生产这种蛋白质，并重新获得能力进行形态分化时，SapB是外源供应。我们现在报告说，大多数的bld突变体被救出SapB生产和气生菌丝体形成时，附近生长的某些其他bld突变体。这种细胞外互补实验表明，形态分化是由至少四种细胞间信号的分级级联决定的。条件培养基中存在至少一种这样的信号。 它耐煮沸和蛋白酶处理，即使在新鲜培养基中稀释至8倍仍有效。
The formation of an aerial mycelium by the filamentous bacterium Streptomyces coelicolor is determined in part by a small morphogenetic protein called SapB. A collection of representative bald (bld) mutants, which are blocked in aerial mycelium formation, are all defective in the production of this protein and regain the capacity to undergo morphological differentiation when SapB is supplied exogenously. We now report that most of the bld mutants are rescued for SapB production and aerial mycelium formation when grown near certain other bld mutants. Extracellular complementation experiments of this kind indicate that morphological differentiation is governed by a hierarchical cascade of at least four kinds of intercellular signals. At least one such signal is present in conditioned medium. It is resistant to boiling and protease treatment, and it remains effective even when diluted up to eightfold in fresh medium.