CHARACTERIZATION AND PRESUMPTIVE IDENTIFICATION OF HELICOBACTER-PYLORI ISOLATES FROM RHESUS-MONKEYS
CHARACTERIZATION AND PRESUMPTIVE IDENTIFICATION OF HELICOBACTER-PYLORI ISOLATES FROM RHESUS-MONKEYS
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DOI：
10.1128/jcm.32.7.1799-1804.1994
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发表时间：
1994-07-01
影响因子：
9.4
通讯作者：
HOLMES, RK
中科院分区：
文献类型：
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作者：
DRAZEK, ES;DUBOIS, A;HOLMES, RK
我们对 38 种螺杆菌分离株进行了鉴定，其中 22 种来自 14 只恒河猴的胃活检样本，以及来自不同群体的 16 只猴子的单一分离株。这些分离株的生化特征与幽门螺杆菌 ATCC 43504 几乎相同。限制性片段长度多态性 (RFLP) 分析表明，每只受感染的猴子都携带一到四个菌株。在这 22 个分离株中发现的 17 个 RFLP 类型与在其他 16 个分离株中发现的所有 7 个 RFLP 类型不同。因此，与来自不同群体的猴子相比，给定群体内的猴子更有可能被具有相同或相似RFLP的螺杆菌分离株感染。通过 PCR 扩增并从恒河猴 85D08 的螺杆菌分离株中克隆出 16S rRNA 基因。使用该探针进行核糖分型表明，恒河猴分离株的多样性低于人类幽门螺杆菌分离株的多样性，对 ureA-ureB 基因簇 PCR 片段的 RFLP 分析也是如此。确定了克隆的 16S rRNA 基因的 DNA 序列，并与报道的幽门螺杆菌和其他螺杆菌物种的序列进行了比较。我们对 127 个核苷酸（对应于大肠杆菌 16S rRNA 基因的残基 1240 至 1366）的分析表明，来自猴 85D08 的幽门螺杆菌分离株与来自人类的幽门螺杆菌分离株有 99.2% 至 100% 同源性，但与该区域的其他幽门螺杆菌物种只有 83.5% 至 96.9% 同源性。 16S rRNA 基因。这些数据为这些分离株被推定为幽门螺杆菌提供了强有力的支持。
We characterized 38 Helicobacter isolates, including 22 from gastric biopsy samples obtained from 14 rhesus monkeys and single isolates from 16 monkeys in a different colony. Biochemical profiles of these isolates were nearly identical to that of Helicobacter pylori ATCC 43504. Restriction fragment length polymorphism (RFLP) analysis indicated that each infected monkey harbored one to four strains. The 17 RFLP types found among these 22 isolates differed from all seven RFLPs found among the other 16 isolates. Thus, monkeys within a given colony are more likely to be infected by Helicobacter isolates with the same or a similar RFLP than are monkeys from different colonies. A 16S rRNA gene was amplified by PCR and cloned from the Helicobacter isolate from rhesus monkey 85D08. Ribotyping with this probe demonstrated less diversity among isolates from rhesus monkeys than was reported among isolates of H. pylori from humans, as did RFLP analysis of a PCR fragment of the ureA-ureB gene cluster. The DNA sequence of the cloned 16S rRNA gene was determined and compared with sequences reported for H. pylori and other Helicobacter species. Our analysis of 127 nucleotides (corresponding with residues 1240 to 1366 of the Escherichia coli 16S rRNA gene) indicated that the Helicobacter isolate from monkey 85D08 was 99.2 to 100% homologous to isolates of H, pylori from humans but only 83.5 to 96.9% homologous with other Helicobacter species in this region of the 16S rRNA gene. These data provide strong support for the presumptive identification of these isolates as H. pylori.