Mutations in the pho80 gene confer permeability to 5'-mononucleotides in Saccharomyces cerevisiae.
Mutations in the pho80 gene confer permeability to 5'-mononucleotides in Saccharomyces cerevisiae.
复制标题
pho80 基因的突变赋予酿酒酵母 5-单核苷酸的通透性。
DOI：
10.1093/genetics/102.3.341
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发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
Thorner,J
中科院分区：
文献类型：
--
作者：
Bisson,LF;Thorner,J
筛选出了dTMP（dTMP）通透的酵母突变株，并鉴定出两个新的互补群（tup 5和tup 7）。在抑制（5 mm PO 4 -3）或去抑制（0.03 mm PO 4 -3）条件下生长的细胞中酸性和碱性磷酸酶水平的测定表明，一般而言，突变导致细胞在磷酸酶合成的调节中有缺陷。此外，3个突变体（tup 1，tup 4和tup 7）显示无机磷转运率显著升高。tup 7位点被发现是紧密的着丝粒连锁的右臂上的染色体XV，并被证明是等位基因与pho 80调节位点的基础上的遗传和生化标准。对已知影响磷酸酶水平（pho）的其他突变的分析表明，有些突变还赋予了dTMP的渗透性。可能的等位基因之间的关系和某些thesephomutations进行了讨论。无论培养条件如何，野生型菌株对dTMP是不可渗透的;相反，在本工作的过程中发现，正常酵母细胞对dUMP是可渗透的，并且dUMP的渗透性受用于生长细胞的培养基中存在的无机磷酸盐的浓度调节。因此，对5′-单甘肽的渗透性似乎受到磷酸酶合成的协调控制。
Yeast mutants permeable to dTMP (tup) were selected and two new complementation groups (tup5andtup7) were identified. Assay of the levels of both acid and alkaline phosphatase in cells grown under either repressing (5 mm PO4-3) or derepressing (0.03 mm PO4-3) conditions indicated that, in general,tupmutations cause cells to be defective in their regulation of phosphatase synthesis. In addition, three of thetupmutations (tup1, tup4andtup7) displayed markedly elevated rates of inorganic phosphate transport. Thetup7locus was found to be tightly centromere-linked on the right arm of chromosomeXV, and was shown to be allelic with thepho80regulatory locus on the basis of both genetic and biochemical criteria. Analysis of other mutations known to affect phosphatase levels (pho) indicated that some also conferred permeability to dTMP. Possible allelic relationships betweentupgenes and certain of thesephomutations are discussed. Regardless of the culture conditions, wild-type strains were not permeable to dTMP; in contrast, it was found in the course of this work that normal yeast cells were permeable to dUMP and that dUMP permeability was regulated by the concentration of inorganic phosphate present in the medium used to grow the cells. Thus, permeability to 5′-mononucleotides appears to be under coordinate control with phosphatase synthesis.