Studies of the distribution of proteins bound to CNBr-activated Sepharose 6B at the electron-microscopic level.
Studies of the distribution of proteins bound to CNBr-activated Sepharose 6B at the electron-microscopic level.
复制标题
在电子显微镜水平上研究与 CNBr 激活的 Sepharose 6B 结合的蛋白质的分布。
DOI：
10.1111/j.1432-1033.1975.tb20988.x
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
R. Koelsch
中科院分区：
文献类型：
--
作者：
J. Lasch;M. Iwig;R. Koelsch
通过CNBr方法将蛋白共价连接到Sepharose上。在电子显微镜水平上研究了它们在载珠上的分布。方法是对凝胶珠进行染色和切片。铁蛋白要么直接偶联到载体的多糖骨架上，要么与纯兔抗氨基肽酶偶联，通过免疫铁蛋白技术可视化共价结合的亮氨酸氨基肽酶。如果满足一些条件，结果证实了早期荧光显微镜下均匀蛋白质分布的发现。
Proteins were covalently attached to Sepharose by the CNBr method. Their distribution across the carrier beads was studied at the electron microscopic level. The approach has been to ferritinstain and to section the gel beads. Ferritin was either coupled directly to the polysaccharide backbone of the carrier or conjugated with pure rabbit anti-aminopeptidase in order to visualize covalently bound leucine aminopeptidase by the immunferritin technique. The results corroborate earlier fluorescence microscopic findings of a uniform protein distribution, provided that a number of conditions are fulfilled.