Gold nanoparticles for high-throughput genotyping of long-range haplotypes
Gold nanoparticles for high-throughput genotyping of long-range haplotypes
复制标题
用于长范围单倍型高通量基因分型的金纳米颗粒
DOI：
10.1038/nnano.2011.141
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发表时间：
2011-10-01
影响因子：
38.3
通讯作者：
Shi, Yongyong
中科院分区：
文献类型：
--
作者：
Chen, Peng;Pan, Dun;Shi, Yongyong
人类基因组计划（1）和人类基因组单体型图计划（2）的完成导致了对人类复杂特征的绘图需求的增加，以了解疾病的病因（3）。识别DNA序列中的变异，这会影响我们如何发展疾病和对病原体和药物的反应，对于这一目的很重要，但很难在大样本集中识别这些变异（3-5）。在这里，我们表明，通过毛细管测序和聚合酶链反应的结合，由金纳米粒子辅助，有可能在人类基因组DNA的重要区域识别与年龄相关性黄斑变性（6-8）和银屑病（9）相关的几种DNA变异。我们的方法是准确的，并且有希望用于对疾病和耐药性的易感性的大规模和高通量遗传分析（10-12）。
Completion of the Human Genome Project(1) and the HapMap Project(2) has led to increasing demands for mapping complex traits in humans to understand the aetiology of diseases(3). Identifying variations in the DNA sequence, which affect how we develop disease and respond to pathogens and drugs, is important for this purpose, but it is difficult to identify these variations in large sample sets(3-5). Here we show that through a combination of capillary sequencing and polymerase chain reaction assisted by gold nanoparticles, it is possible to identify several DNA variations that are associated with age-related macular degeneration(6-8) and psoriasis(9) on significant regions of human genomic DNA. Our method is accurate and promising for large-scale and high-throughput genetic analysis of susceptibility towards disease and drug resistance(10-12).