Comparative analysis of epicuticular lipid profiles of sympatric and allopatric field populations of Anopheles gambiae s.s. molecular forms and An. arabiensis from Burkina Faso (West Africa)
Comparative analysis of epicuticular lipid profiles of sympatric and allopatric field populations of Anopheles gambiae s.s. molecular forms and An. arabiensis from Burkina Faso (West Africa)
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DOI：
10.1016/j.ibmb.2007.01.002
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发表时间：
2007-04-01
影响因子：
3.8
通讯作者：
della Torre, A.
中科院分区：
文献类型：
--
作者：
Caputo, B.;Dani, F. R.;della Torre, A.
我们分析了气相色谱-质谱法（GC-MS）和气相色谱-火焰离子化检测器（GC-FID）的上表皮脂质配置文件的主要非洲热带疟疾媒介，冈比亚按蚊领域的女性。这些样本是在布基纳法索（西非）的三个村庄收集的，那里有一个。gambiae M和S分子型和An. Arabiensis同域生活。目的是比较这三个类群的个别野外标本的表皮碳氢化合物（CHC）组成，以突出它们之间可能存在的差异。通过GC-MS分析的所有样品（55个个体和8个池）的特征在于相同的48个CHCs和10个含氧化合物。通过GC-FID对174份样本中19种最丰富的CHCs进行了定量：发现两种AN的异域种群之间存在定量的分类群内差异。arabiensis和S型。还发现了同域分类对之间某些碳氢化合物相对丰度的分类间数量差异，这似乎主要与当地情况有关，可能除了An之间的diMeC（35）。arabiensis和S型。此外，MeC 29在所有三个村庄的S型和M型之间显示出一定程度的分化。这些差异的可能原因进行了讨论。(c)2007爱思唯尔有限公司保留所有权利。
We analysed by gas chromatography-mass spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID) the epicuticular lipid profiles of field females of the major Afro-tropical malaria vector, Anopheles gambiae. The samples were collected in three villages in Burkina Faso (West Africa), where An. gambiae M and S molecular forms and An. arabiensis live sympatrically. The aim was to compare the cuticular hydrocarbon (CHC) composition of individual field specimens of these three taxa, to highlight possible differences among them. All the samples analysed by GC-MS (55 individuals and eight pools) were characterized by the same 48 CHCs and 10 oxygenated compounds. The 19 most abundant CHCs were quantified in 174 specimens by GC-FID: quantitative intra-taxon differences were found between allopatric populations of both An. arabiensis and S-form. Inter-taxa quantitative differences in the relative abundances of some hydrocarbons between pairs of sympatric taxa were also found, which appear to be mainly linked to local situations, with the possible exception of diMeC(35) between An. arabiensis and S-form. Moreover, MeC29 shows some degree of differentiation between S- and M-form in all three villages. Possible causes of these differences are discussed. (c) 2007 Elsevier Ltd. All rights reserved.