Current topics in RNA-protein recognition: Control of specificity and biological function through induced fit and conformational capture
Current topics in RNA-protein recognition: Control of specificity and biological function through induced fit and conformational capture
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DOI：
10.1021/bi010680y
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发表时间：
2001-07-10
期刊：
影响因子：
2.9
通讯作者：
Varani, G
中科院分区：
文献类型：
--
作者：
Leulliot, N;Varani, G
当RNA和蛋白质相互结合时，识别几乎总是通过“诱导配合”而不是通过刚性的“锁和钥匙”对接发生（1，2）。蛋白质，RNA，有时两者都经历大的构象变化，并在复合物形成过程中捕获次要的种群物种，导致相互作用组分的局部和宏观性质发生大的变化。虽然这些构象转换已被观察到在几乎所有的RNA-蛋白质复合物研究的日期，没有统一的图片已经出现关于它们的相互作用的亲和力和特异性或构象适应性的功能作用的含义。RNA-蛋白质复合物的表征以及蛋白质折叠和蛋白质识别研究中概念的应用的最新进展为RNA-蛋白质识别提供了新的见解。
When RNA and protein bind each other, recognition occurs almost invariably by “induced fit” rather than by rigid “lock-and-key” docking (1, 2). The protein, the RNA, and sometimes both undergo large conformational changes, and minor populated species are captured during complex formation, leading to large changes in the local as well as macroscopic properties of the interacting components. Although these conformational transitions have been observed in almost all RNA-protein complexes studied to date, no unifying picture has emerged concerning their implication in the affinity and specificity of the interaction or the functional role of conformational adaptability. Recent advances in the characterization of RNA-protein complexes and the application of concepts derived from protein folding and protein recognition studies are providing new insight into RNA-protein recognition.