Different expression of Lyt differentiation antigens and cell surface glycoproteins by a murine T lymphoma line and its highly metastatic variant
Different expression of Lyt differentiation antigens and cell surface glycoproteins by a murine T lymphoma line and its highly metastatic variant
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鼠T淋巴瘤系及其高度转移变体对Lyt分化抗原和细胞表面糖蛋白的不同表达
DOI：
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发表时间：
1982
影响因子：
5.4
通讯作者：
V. Schirrmacher
中科院分区：
文献类型：
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作者：
P. Altevogt;J. Kurnick;A. Kimura;K. Bosslet;V. Schirrmacher
分析了甲基胆蒽诱导的DBA/2 T淋巴瘤Eb及其高转移性变体系ESb的克隆系在血清学可检测的细胞表面分化标志物表达方面的差异。对用异硫氰酸荧光素偶联的单克隆大鼠抗小鼠Thy-1、Lyt-1、Lyt-2染色的细胞和用小鼠抗Lyt-3.2和Lyt-6.2的同种异体抗血清进行的补体依赖性细胞毒性的流式细胞荧光分析显示，亲本低转移系Eb的表型为Thy-1+、Lyt-1-、Lyt-2+、Lyt-3+、Lyt-6+，而高转移变异系的表型为Thy-1-、Lyt-1+、Lyt-2-、Lyt-3−，Ly-6−.在十二烷基硫酸钠存在下，通过聚丙烯酰胺凝胶电泳对Eb和ESB克隆的半乳糖氧化酶/NaB 3 H4标记糖蛋白进行分析，结果显示了进一步的表型差异。放射性标记的糖蛋白分别选择性结合到H. pomatia或Vicia villosa-琼脂糖凝胶上，允许鉴定在Hb细胞上表达的T130和在一些ESb克隆上表达的T145。后一种抗原在鼠细胞毒性T淋巴细胞上表达。免疫沉淀分析表明，Eb和ESb携带不同分子形式的T200抗原。来自肿瘤细胞的碘化表面蛋白的比较，无论是治疗或未治疗的衣霉素表明，许多的Eb和ESB细胞之间的膜蛋白的差异可以归因于糖基化的差异。我们的研究结果，来自一个确定的肿瘤系统的淋巴起源，表明从低到高恶性肿瘤系的进展可以与各种定义的细胞表面分化抗原的表达的变化。肿瘤进展和细胞分化或去分化之间可能存在的关系的问题进行了讨论。
Cloned lines of the methylcholanthrene‐induced DBA/2 T lymphoma Eb and its highly metastatic variant line ESb were analyzed for differences in the expression of serologically detectable cell surface differentiation markers. Flow cytofluorographic analysis of cells stained with fluorescein isothiocyanate‐conjugated monoclonal rat anti‐mouse Thy‐1, Lyt‐1, Lyt‐2 and compelement‐dependent cytotoxicity with mouse alloantisera against Lyt‐3.2 and Ly‐6.2 revealed, for the parental low metastasizing line, Eb, a phenotype of Thy‐l+, Lyt‐1−, Lyt‐2+, Lyt‐3+, Ly‐6+, whereas the highly metastasizing variant line typed as Thy‐1−, Lyt‐1+, Lyt‐2−, Lyt‐3−, Ly‐6−. Analysis of galactose oxidase/NaB3H4‐labeled glycoproteins from Eb and ESb clones by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate showed further phenotypic differences. Selective binding of radiolabeled glycoproteins to Helix pomatia or Vicia villosa‐Sepharose, respectively, allowed the identification of T130 to be expressed on Hb cells and T145 to be expressed on some ESb clones. The latter antigen is expressed on murine cytotoxic T lymphocytes. Immune precipitation analysis revealed that Eb and ESb bear different molecular forms of the T200 antigen. Comparisons of iodinated surface proteins derived from tumor cells either treated or untreated with tunicamycin indicated that many of the differences in membrane proteins between Eb and ESb cells could be attributed to differences in glycosylation. Our results, derived from a defined tumor system of lymphoid origin, show that the progression from a low to a high malignant tumor line can be associated with changes in the expression of various defined cell surface differentiation antigens. The question of a possible relationship between tumor progression and cell differentiation or dedifferentiation is discussed.