Carbonic anhydrase is not a relevant nitrite reductase or nitrous anhydrase in the lung
Carbonic anhydrase is not a relevant nitrite reductase or nitrous anhydrase in the lung
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DOI：
10.1113/jp275894
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发表时间：
2018-05
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通讯作者：
P. Pickerodt;S. Kronfeldt;M. Russ;A. González-López;P. Lother;Elvira Steiner;K. Vorbrodt;T. Busch;W. Boemke;R. Francis;E. Swenson
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作者：
P. Pickerodt;S. Kronfeldt;M. Russ;A. González-López;P. Lother;Elvira Steiner;K. Vorbrodt;T. Busch;W. Boemke;R. Francis;E. Swenson
碳酸酐酶(CA)抑制剂，如乙酰唑胺，可抑制人类和其他哺乳动物的缺氧性肺血管收缩(HPV)，但其作用机制尚不清楚。据推测，碳酸酐酶在体内可能作为一种亚硝酸脱水酶来从亚硝酸盐中生成一氧化氮(NO)，而且这种形成在乙酰唑胺存在时会增加。在没有任何NO生成证据的情况下，乙酰唑胺降低了猪的HPV，而雾化吸入的亚硝酸钠通过NO的形成减少了HPV；然而，在暴露于肺泡低氧的猪中，联合注入乙酰唑胺和亚硝酸钠并不会进一步增加呼出的NO浓度。我们的结论是，在猪和其他哺乳动物的肺中，乙酰唑胺既不是亚硝酸脱水酶，也不是亚硝酸盐还原酶，从而解释了它在肺或全身循环中的血管扩张作用。
Carbonic anhydrase (CA) inhibitors such as acetazolamide inhibit hypoxic pulmonary vasoconstriction (HPV) in humans and other mammals, but the mechanism of this action remains unknown. It has been postulated that carbonic anhydrase may act as a nitrous anhydrase in vivo to generate nitric oxide (NO) from nitrite and that this formation is increased in the presence of acetazolamide. Acetazolamide reduces HPV in pigs without evidence of any NO generation, whereas nebulized sodium nitrite reduces HPV by NO formation; however; combined infusion of acetazolamide with sodium nitrite inhalation did not further increase exhaled NO concentration over inhaled nitrite alone in pigs exposed to alveolar hypoxia. We conclude that acetazolamide does not function as either a nitrous anhydrase or a nitrite reductase in the lungs of pigs, and probably other mammals, to explain its vasodilating actions in the pulmonary or systemic circulations.