Co-expression of vasoactive intestinal peptide and protein gene product 9.5 surrounding the lumen of human Schlemm's canal
Co-expression of vasoactive intestinal peptide and protein gene product 9.5 surrounding the lumen of human Schlemm's canal
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DOI：
10.1016/j.exer.2018.02.005
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发表时间：
2018-05-01
影响因子：
3.4
通讯作者：
Zhang, Hong
中科院分区：
文献类型：
--
作者：
Ji, Pingting;Chen, Liwen;Zhang, Hong
以前对房水流出的研究主要集中在小梁网（TM）的阻力上，而对施累姆氏管（SC）的功能知之甚少。在这里，我们的目的是研究 SC 是否受周围神经系统支配。对来自八位捐赠者的十份眼睛样本进行了组织学分析。 CD31用于识别SC，之后我们使用蛋白质基因产物（PGP）9.5作为标记来检测SC周围的神经纤维。然后，我们通过 PGP9.5 和交感神经标记物双染色来表征神经，例如酪氨酸羟化酶 (TH) 和多巴胺 β-羟化酶 (D beta H)，或副交感神经标记物血管活性肠肽 (VIP)，以及感觉神经标记物降钙素基因相关肽 (CGRP) 和囊泡谷氨酸转运蛋白 2 (VGLUT2)。免疫组织化学和免疫荧光检测SC中γ上皮Na+通道（γ-ENaC）的表达。我们发现前房角表达了不同的标记物，SC 的管腔表面仅 PGP9.5、VIP 和 γ-ENaC 呈阳性染色。 CGRP 和 VGLUT2 在 TM 和巩膜刺 (SS) 中表达，而 TH 和 D beta H 在 TM 和 SC 中均不表达。此外，PGP9.5 在 SC 周围区域以及 SS 中与 VIP 和 γ-ENaC 共表达。我们的研究结果表明，周围神经在解剖学上分布在 SC 周围的组织中，局部神经纤维可能是副交感神经或感觉神经纤维，而不是交感神经纤维。
Previous studies of aqueous humor outflow have focused primarily on resistance at the trabecular meshwork (TM), and little is known about the function of Schlemm's canal (SC). Here, we aimed to investigate whether SC is innervated by the peripheral nervous system. Ten eye specimens from eight donors were processed for histological analysis. CD31 was used to identify SC, after which we used protein gene product (PGP) 9.5 as a marker to detect nerve fibers around SC. We then characterized the nerves by double staining for PGP9.5 and sympathetic nerve markers, such as tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (D beta H), or the parasympathetic marker vasoactive intestinal peptide (VIP), as well as sensory nerve marker calcitonin gene-related peptide (CGRP) and vesicular glutamate transporter 2 (VGLUT2). Immunohistochemistry and immunofluorescence were also performed to detect the expression of gamma-epithelial Na+ channel (gamma-ENaC) in SC. We found that different markers were expressed in the anterior chamber angle, with the luminal surface of SC were only positive stained for PGP9.5, VIP, and gamma-ENaC. CGRP and VGLUT2 were expressed in TM and scleral spur (SS), whereas TH and D beta H were absent in both TM and SC. Furthermore, PGP9.5 was co-expressed with VIP and gamma-ENaC in the region surrounding the SC as well as in SS. Our findings indicate that the peripheral nerves anatomically spread in the tissues around the SC and the local nerve fibers may be parasympathetic or sensory rather than sympathetic.