The evaluation of lipid peroxidation and adenosine deaminase activity in patients with Behcet's disease
The evaluation of lipid peroxidation and adenosine deaminase activity in patients with Behcet's disease
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DOI：
10.1016/s0009-9120(01)00190-4
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发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Asçioglu, Ö
中科院分区：
文献类型：
--
作者：
Köse, K;Yazici, C;Asçioglu, Ö
目的：尽管病因不明，免疫学改变和中性粒细胞功能亢进可能参与白塞病（BD）的发病机制。本研究的目的是探讨腺苷脱氨酶（ADA）活性作为T淋巴细胞活化的非特异性标志物是否可能在ED中起潜在作用，以及是否可能与中性粒细胞产生活性氧（ROS）有关。在25例BD患者和25例健康对照者中，采用荧光定量法测定血浆和红细胞中的MDA（ROS诱导的脂质过氧化的终产物）水平。血浆ADA活性升高，红细胞ADA活性降低，艾德患者血浆和红细胞MDA水平均高于对照组。此外，血浆ADA活性与血浆（p < 0.05）和红细胞（p < 0.01）中MDA水平呈正相关。MDA含量与肝硬化程度呈正相关（p < 0.05），但ADA活性之间呈负相关（p < 0.01），红细胞中ADA和MDA值之间也存在差异艾德组与对照组比较差异有显著性（p < 0.01）。这些发现可能为T淋巴细胞活化在艾德中的潜在作用提供了一些证据，如血浆ADA活性增加所反映的，以及活化的T细胞和中性粒细胞功能亢进之间可能存在的相互关系。如ROS生成，如MDA水平增加所反映的。(C)2001年加拿大临床化学家协会。All rights reserved.
Objective: Despite unknown etiology, immunologic alterations and neutrophil hyperfunctions may be involved in the etiopathogenesis of Behcet's Disease (BD). The purpose of the study was to investigate whether adenosine deaminase (ADA) activity, accepted as a nonspecific marker of T lymphocyte activation, may have a potential role in ED. and also may be related to the production of reactive oxygen species (ROS) by neutrophils.Design and methods: ADA activities and malondialdehyde (MDA; endproduct of lipid peroxidation induced by ROS) levels in both plasma and erythrocytes were spectrophotometrically measured in 25 patients with BD and also in 25 healthy controls.Results: ADA activity was found to be higher in plasma, but lower in erythrocytes; plasma and erythrocyte MDA levels were higher in ED patients than those of controls. In addition, plasma ADA activity was positively related to MDA levels in both plasma (p < 0.05) and erythrocytes (p < 0.01). There was also positive correlation between MDA levels (p < 0.05), but negative correlations between ADA activities (p < 0.01) and also between ADA and MDA values in erythrocytes (p < 0.01) of ED patients.Conclusion: These findings may provide some evidence for a potential role of T lymphocyte activation in ED as reflected by increased plasma ADA activity, and for the presence of possible interrelationship between activated T cells and neutrophil hyperfunctions. such as ROS generation, as reflected by increased MDA levels. (C) 2001 The Canadian Society of Clinical Chemists. All rights reserved.