STUDIES ON THE POSSIBLE INHIBITORY EFFECT OF CORTICOSTERONE ON CORTICOSTEROIDOGENESIS AT THE ADRENAL LEVEL IN THE RAT
STUDIES ON THE POSSIBLE INHIBITORY EFFECT OF CORTICOSTERONE ON CORTICOSTEROIDOGENESIS AT THE ADRENAL LEVEL IN THE RAT
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DOI：
10.1210/endo-67-3-379
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发表时间：
1960-01-01
期刊：
影响因子：
4.8
通讯作者：
DORFMAN, RI
中科院分区：
文献类型：
--
作者：
PERON, FG;MONCLOA, F;DORFMAN, RI
皮下注射5 nag.在大鼠中的皮质酮中，该物质的血浆水平在注射后1和2小时达到最大。尽管循环皮质酮水平高度升高，但肾上腺中未发生这种物质的摄取。在皮下注射后2小时，引起肾上腺皮质酮最大升高所需的ACTH的最佳量为2个单位。同时给予2单位ACTH和5 mg.正常大鼠的平均肾上腺皮质酮低于单独给予1单位ACTH时的水平。当注射4单位ACTH时，平均肾上腺皮质酮显著降低（P< 0.01）。用ACTH或ACTH加皮质酮预处理的垂体切除大鼠重复这些实验表明，ACTH的皮质类固醇生成作用降低。大鼠肾上腺切片在体外与20 μ g肾上腺素孵育时，未观察到ACTH反应。皮质酮和50毫单位促肾上腺皮质激素实验表明，大鼠血液中皮质酮的循环水平可能通过抑制ACTH的作用直接作用于肾上腺来控制其产生。
Following the subcutaneous administration of 5 nag. of corticosterone in the rat, the plasma level of this substance was maximal 1 and 2 hours following the injection. In spite of the highly elevated level of circulating corticosterone, no uptake of this substance occurred in the adrenals. The optimal amount of ACTH required to elicit a maximum elevation of adrenal corticosterone two hours after its subcutaneous injection was 2 units. Two hours after the simultaneous administration of 2 units of ACTH and 5 mg. of corticosterone in the intact rat the mean adrenal corticosterone was lower than when 1 units of ACTH were administered alone. When the experiment was repeated with 4 units of ACTH being injected the mean adrenal corticosterone was significantly lower (P< 0.01). Repetition of these experiments with ACTH or ACTH plus corticosterone-pretreated hypophysectomized rats indicated that the corticosteroidogenic effect of ACTH was decreased. No ACTH response was observed in rat adrenal sections when these were incubated in vitro with 20 ug. of corticosterone and 50 milliunits of ACTH. The experiments suggest that the circulating levels of blood corticosterone in the rat may control its production by acting directly on the adrenal gland by inhibiting the action of ACTH.