Orientation of microtubules suggests a role in mRNA transportation in fertilized eggs of Chinese pine (Pinus tabulaeformis)
Orientation of microtubules suggests a role in mRNA transportation in fertilized eggs of Chinese pine (Pinus tabulaeformis)
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微管的方向表明在油松受精卵 mRNA 运输中的作用
DOI：
10.1007/s00709-007-0266-7
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发表时间：
2007-10-01
期刊：
影响因子：
2.9
通讯作者：
Han, Yuzhen
中科院分区：
文献类型：
--
作者：
Guo, Fengli;Yu, Lifeng;Han, Yuzhen
油松受精后卵细胞中的多核糖体与微管结合，为微管的胞内mRNA和多核糖体定位提供了直接证据。我们用免疫电镜研究了MT在受精卵细胞中的定位和空间分布，以及它们与多核糖体的关系。受精后不久，受精卵中的MT和多聚核糖体在核周积累。此时，一些MT垂直于核膜，一端直接连接到外膜或核孔复合体（NPC），另一端到达外层或皮质MT，与合子的长轴平行。核周区的多核糖体显示出与MT相同的空间和时间模式。mRNA结合蛋白hnRNP的免疫定位表明，mRNA从核孔复合物出口后立即加载到核相关的MT上。然后，多核糖体和mRNA从这些MT转运到外层和/或皮质MT，在那里它们进一步定位到细胞的极区。
Polysomes become associated with microtubules (MTs) in egg cells of Chinese pine upon fertilization, providing direct evidence for MT-based intracellular mRNA and polysome localization. We have investigated by immunoelectron microscopy the orientation and spatial distribution of MTs and their association with polysomes in the fertilized egg cells. There is a perinuclear accumulation of MTs and polysomes in the zygote soon after fertilization. At this time, some of the MTs are perpendicular to the nuclear envelope and directly connected to the outer membrane or nuclear-pore complexes (NPC) at one end, and the other ends reach to the outer tier or cortical MTs that are parallel to the long axis of the zygote. The polysomes in the perinuclear region show the same spatial and temporal pattern as the MTs. Immunolocalization of the mRNA-binding protein hnRNP indicates that the mRNAs are loaded onto the nucleus-associated MTs immediately after their export from the nuclear-pore complexes. The polysomes and mRNAs are then transported from these MTs to the outer tier and/or cortical MTs, where they further localize to the polar region of the cell.