NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats
NMR-Based Metabonomic Study Reveals Intervention Effects of Polydatin on Potassium Oxonate-Induced Hyperuricemia in Rats
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DOI：
10.1155/2020/6943860
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发表时间：
2020-07-06
影响因子：
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通讯作者：
Zou, Zhongjie
中科院分区：
文献类型：
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作者：
Han, Bin;Gong, Mengjuan;Zou, Zhongjie
白藜芦醇的天然前体白藜芦醇苷具有抗高尿酸血症的作用，但其作用机制尚不清楚。本研究旨在通过代谢组学技术从整体角度评价虎杖苷对氧磷酸钾诱导的大鼠高尿酸血症的治疗作用及其机制。应用核磁共振（NMR）光谱法，以捕捉从高尿酸血症和虎杖苷治疗诱导的大鼠收集的血清和尿液中的代谢变化。多变量分析显示，高尿酸血症大鼠与健康对照组相比，代谢紊乱明显。在大鼠血清和尿液中分别鉴定出11种和6种代谢产物为与高尿酸血症相关的差异代谢产物。这些途径主要包括支链氨基酸（BCAA）代谢、糖酵解、三羧酸循环、酮体的合成和降解、嘌呤代谢和肠道菌群代谢。此外，一些代谢物表明高尿酸血症诱导的肾损伤风险。虎杖苷能显著降低高尿酸血症大鼠的血尿酸、肌酐和血尿素氮水平，并通过部分恢复紊乱的代谢途径的平衡，缓解异常的代谢状态。本研究结果有助于深入了解高尿酸血症的病理生理过程，为揭示虎杖苷治疗高尿酸血症的代谢机制提供参考。
Previous studies have disclosed the antihyperuricemic effect of polydatin, a natural precursor of resveratrol; however, the mechanisms of action still remain elusive. The present study was undertaken to evaluate the therapeutic effects and the underlying mechanisms of polydatin on potassium oxonate-induced hyperuricemia in rats through metabonomic technology from a holistic view. Nuclear magnetic resonance (NMR) spectroscopy was applied to capture the metabolic changes in sera and urine collected from rats induced by hyperuricemia and polydatin treatment. With multivariate data analysis, significant metabolic perturbations were observed in hyperuricemic rats compared with the healthy controls. A total of eleven and six metabolites were identified as differential metabolites related to hyperuricemia in serum and urine of rats, respectively. The proposed pathways primarily included branched-chain amino acid (BCAA) metabolism, glycolysis, the tricarboxylic acid cycle, synthesis and degradation of ketone bodies, purine metabolism, and intestinal microflora metabolism. Additionally, some metabolites indicated the risk of renal injury induced by hyperuricemia. Polydatin significantly lowered the levels of serum uric acid, creatinine, and blood urea nitrogen and alleviated the abnormal metabolic status in hyperuricemic rats by partially restoring the balance of the perturbed metabolic pathways. Our findings shed light on the understanding of the pathophysiological process of hyperuricemia and provided a reference for revealing the metabolic mechanism produced by polydatin in the treatment of hyperuricemia.