Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography.
Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography.
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DOI：
10.1038/srep22079
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发表时间：
2016-02-25
影响因子：
4.6
通讯作者：
Cho SH
中科院分区：
文献类型：
--
作者：
Manohar N;Reynoso FJ;Diagaradjane P;Krishnan S;Cho SH
X射线荧光计算机断层扫描（XFCT）是一种通过检测由激发源（通常来自同步加速器）激发的X射线荧光（特征X射线）来识别、量化和定位物体内元素的技术。然而，同步加速器的使用限制了XFCT用于常规生物医学成像应用的实用性和可访问性。因此，我们已经开发出在台式设置上使用普通多色X射线源执行XFCT的能力。在这里，我们报告了我们的尸检研究，证明了使用台式XFCT准确成像金纳米粒子（GNP）注射到荷瘤小鼠的分布。使用电感耦合等离子体质谱法验证了台式XFCT测定的GNPs分布。这项研究表明，大幅提高灵敏度和特异性的GNP检测和定量与台式XFCT，高达两个数量级优于传统的X射线CT。结果还重申了台式XFCT在小动物成像背景下同时测定非放射性金属探针（如GNP）的空间分布和浓度的独特能力。总体而言，这项调查确定了一个明确的路径，在体内分子成像使用台式XFCT技术结合GNP和其他金属探针。
X-ray fluorescence computed tomography (XFCT) is a technique that can identify, quantify, and locate elements within objects by detecting x-ray fluorescence (characteristic x-rays) stimulated by an excitation source, typically derived from a synchrotron. However, the use of a synchrotron limits practicality and accessibility of XFCT for routine biomedical imaging applications. Therefore, we have developed the ability to perform XFCT on a benchtop setting with ordinary polychromatic x-ray sources. Here, we report our postmortem study that demonstrates the use of benchtop XFCT to accurately image the distribution of gold nanoparticles (GNPs) injected into a tumor-bearing mouse. The distribution of GNPs as determined by benchtop XFCT was validated using inductively coupled plasma mass spectrometry. This investigation shows drastically enhanced sensitivity and specificity of GNP detection and quantification with benchtop XFCT, up to two orders of magnitude better than conventional x-ray CT. The results also reaffirm the unique capabilities of benchtop XFCT for simultaneous determination of the spatial distribution and concentration of nonradioactive metallic probes, such as GNPs, within the context of small animal imaging. Overall, this investigation identifies a clear path toward in vivo molecular imaging using benchtop XFCT techniques in conjunction with GNPs and other metallic probes.