Spectral-domain phase microscopy
Spectral-domain phase microscopy
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DOI：
10.1364/ol.30.001162
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发表时间：
2005-05-15
期刊：
影响因子：
3.6
通讯作者：
Izatt, JA
中科院分区：
文献类型：
--
作者：
Choma, MA;Ellerbee, AK;Izatt, JA
宽带干涉术是一种有吸引力的技术，用于检测细胞的运动，因为它提供了深度分辨相位信息通过相干门。我们提出了一种相位敏感的技术称为光谱域相位显微镜（SDPM）。SDPM是光谱域光学相干断层扫描的功能扩展，允许检测活细胞中的纳米级运动。证明了该技术的灵敏度，并验证了其校准。散粒噪声限制的位移灵敏度，这种技术的推导。对从鸡胚分离的自发搏动心肌细胞进行细胞动力学测量。(c)2005年，美国光学学会。
Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos. (c) 2005 Optical Society of America.