Lung virome: New potential biomarkers for asthma severity and exacerbation
Lung virome: New potential biomarkers for asthma severity and exacerbation
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DOI：
10.1016/j.jaci.2021.03.017
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发表时间：
2021-10-05
影响因子：
14.2
通讯作者：
Yi, Hana
中科院分区：
文献类型：
--
作者：
Choi, Sungmi;Sohn, Kyoung-Hee;Yi, Hana
背景：虽然已知一些呼吸道病毒感染有助于哮喘的发生和加重，但由于技术上的挑战，呼吸道的共生病毒尚未得到广泛研究。目的：研究哮喘气道中病毒的特征。方法：对12例健康人、15例非重度哮喘患者和15例重度哮喘患者的痰中细菌组和病毒组进行分析，并评估其与严重程度、加重程度、哮喘控制试验（ACT）和肺功能等临床特征的相关性。结果：气道内16S核糖体RNA细菌组分析未发现差异，但健康对照组和哮喘患者气道病毒组的多样性和组成存在明显差异。疱疹病毒是哮喘组中最丰富的病毒类型（44.6 +/- 4.6%），主要是巨细胞病毒（CMV）和EBV，分别占24.5 +/- 3.3%和16.9 +/- 3.5%，而健康对照组分别为5.4 +/- 2.5%和7.1 +/- 3.0%。CMV和EBV在哮喘加重患者中更丰富，且其丰度与哮喘更严重、ACT评分较低、肺功能较低相关。相反，在健康对照中丰富的噬菌体在哮喘患者中按非严重哮喘和严重哮喘的顺序严重减少，并与ACT和FEV1/用力肺活量呈显著正相关。结论：肺病毒组，特别是巨细胞病毒、EBV和噬菌体可能是哮喘严重程度和恶化的潜在生物标志物。
Background: Although some respiratory virus infections are known to contribute to the development and exacerbation of asthma, commensal viromes in airway have not been extensively studied due to technical challenges.Objectives: This study investigated the characteristics of the virome in asthmatic airways.Methods: Both the bacteriome and virome profiles in sputum from 12 healthy individuals, 15 patients with nonsevere asthma, and 15 patients with severe asthma were analyzed and assessed for the association with clinical characteristics such as severity, exacerbation, Asthma Control Test (ACT), and lung function.Results: While analysis of the 16S ribosomal RNA bacteriome in the airway showed no differences, clear contrasts in the diversity and composition of airway viromes were observed between healthy controls and patients with asthma. Herpesviruses were the most abundant type of virus in the asthma group (44.6 +/- 4.6%), mainly with cytomegalovirus (CMV) and EBV accounting for 24.5 +/- 3.3% and 16.9 +/- 3.5%, respectively, in contrast to those in the healthy controls (5.4 +/- 2.5% and 7.1 +/- 3.0%, respectively). CMV and EBV were more abundant in patients with asthma who experienced exacerbation, and their abundance showed correlation with more severe asthma, lower ACT score, and lower lung function. On the contrary, bacteriophage that is abundant in healthy controls was severely reduced in patients with asthma in the order of nonsevere and severe asthma and presented significant positive correlation with ACT and FEV1/forced vital capacity.Conclusions: Lung viromes, especially, CMV, EBV, and bacteriophage may be potential biomarkers of asthma severity and exacerbation.