Nano-formulated curcumin (Lipodisq™) modulates the local inflammatory response, reduces glial scar and preserves the white matter after spinal cord injury in rats
Nano-formulated curcumin (Lipodisq™) modulates the local inflammatory response, reduces glial scar and preserves the white matter after spinal cord injury in rats
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DOI：
10.1016/j.neuropharm.2019.05.018
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发表时间：
2019-09-01
影响因子：
4.7
通讯作者：
Urdzikova, Lucia Machova
中科院分区：
文献类型：
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作者：
Krupa, Petr;Svobodova, Barbora;Urdzikova, Lucia Machova
高水溶性纳米姜黄素用于治疗大鼠脊髓损伤（SCI）实验模型。纳米姜黄素和作为对照的载体纳米载体在脊髓损伤后立即局部递送，并且在SCI后连续4周期间腹膜内递送。使用行为测试评估治疗的功效，所述行为测试在实验期间每周进行，持续9周。行为测试（BBB、平束测试、旋转棒、motoRater）显示与纳米载体对照相比，纳米姜黄素治疗组中的显著改善。在实验结束时进行脊髓组织的免疫组织化学分析，这证明了在纳米姜黄素处理组中白色组织的显著保留、神经胶质瘢痕面积的减少和更高量的新发芽轴突。通过qPCR评估内源性基因（Sort 1、Fgf 2、Irf 5、Mrc 1、Olig 2、Casp 3、Gap 43、GFap、Vegf、Nfk β）和白细胞介素（IL-1 β、TNF-α、IL-6、IL-12、CCL-5、IL-11、IL-10、IL-13）的表达，并显示SCI后前两周内炎性细胞因子表达的变化。
A highly water soluble, nano-formulated curcumin was used for the treatment of the experimental model of spinal cord injury (SCI) in rats. Nanocurcumin and a vehicle nanocarrier as a control, were delivered both locally, immediately after the spinal cord injury, and intraperitoneally during the 4 consecutive weeks after SCI. The efficacy of the treatment was assessed using behavioral tests, which were performed during the experiment, weekly for 9 weeks. The behavioral tests (BBB, flat beam test, rotarod, motoRater) revealed a significant improvement in the nanocurcumin treated group, compared to the nanocarrier control. An immunohistochemical analysis of the spinal cord tissue was performed at the end of the experiment and this proved a significant preservation of the white matter tissue, a reduced area of glial scaring and a higher amount of newly sprouted axons in the nanocurcumin treated group. The expression of endogenous genes (Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, Nfk beta) and interleukins(IL-1 beta, TNF-alpha, IL-6, IL-12, CCL-5, IL-11, IL-10, IL-13) was evaluated by qPCR and showed changes in the expression of the inflammatory cytokines in the first two weeks after SCI.