Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens.
Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens.
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不同NA基因重配H9流感病毒对小鼠和鸡的致病性
DOI：
10.1186/s13567-016-0352-y
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发表时间：
2016-06-24
影响因子：
4.4
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Yan L;Liu Q;Su X;Teng Q;Bao D;Che G;Chen H;Cui H;Ruan T;Li X;Li Z
为了更好地了解不同NA基因对H9病毒致病能力的影响，我们构建了3种重组H9病毒(rH9N1、H9N2和rH9N3)，并对其进行了鉴定。这三种病毒在鸡蛋和MDCK细胞中的复制效率都很高，而rH9N1和rH9N3在A549细胞中的复制效率高于H9N2。RH9N3病毒在小鼠体内的复制效率高于rH9N1和H9N2病毒，而rH9N3病毒在鸡体内的复制和排泄效率低于H9N2病毒。进一步的研究表明，N3具有更高的NA活性和更快的从红细胞释放病毒的速度，这可能改善了H9流感病毒对哺乳动物的适应。
To better understand the influence of different NA genes on pathogenicity of H9 viruses, three reassortant H9 viruses (rH9N1, H9N2 and rH9N3) were generated and characterized. All three viruses replicated efficiently in eggs and MDCK cells, whereas the rH9N1 and rH9N3 replicated more efficiently than H9N2 in A549 cells. The rH9N3 replicated more efficiently than rH9N1 and H9N2 viruses in mice, however, rH9N3 replicated and shed less efficiently than the H9N2 virus in chickens. Further studies indicate that N3 had higher NA activity and released virus from erythrocytes faster, which may improve the adaptation of H9 influenza virus to mammals.