ROLE OF YEAST INSULIN-DEGRADING ENZYME HOMOLOGS IN PROPHEROMONE PROCESSING AND BUD SITE SELECTION
ROLE OF YEAST INSULIN-DEGRADING ENZYME HOMOLOGS IN PROPHEROMONE PROCESSING AND BUD SITE SELECTION
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DOI：
10.1126/science.270.5235.464
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发表时间：
1995-10-20
期刊：
影响因子：
56.9
通讯作者：
BOONE, C
中科院分区：
文献类型：
--
作者：
ADAMES, N;BLUNDELL, K;BOONE, C
酿酒酵母 AXL1 基因产物 Axl1p 与内切蛋白酶的胰岛素降解酶家族具有同源性。酵母 axl1 突变体显示 a 因子信息素分泌缺陷，并且在 a 因子前体中鉴定出 Axl1p 可能的加工位点。此外，Axl1p 似乎充当轴芽位点选择的形态发生决定因素。 Axl1p 假定活性位点内的氨基酸取代导致信息素加工缺陷，但未能干扰芽位点选择。因此，Axl1p 已被证明参与不同信号传导途径的双重调节，并且胰岛素酶家族的成员参与前肽加工。
The Saccharomyces cerevisiae AXL1 gene product Axl1p shares homology with the insulin-degrading enzyme family of endoproteases. Yeast axl1 mutants showed a defect in a-factor pheromone secretion, and a probable site of processing by Axl1p was identified within the a-factor precursor. In addition, Axl1p appears to function as a morphogenetic determinant for axial bud site selection. Amino acid substitutions within the presumptive active site of Axl1p caused defects in propheromone processing but failed to perturb bud site selection. Thus, Axl1p has been shown to participate in the dual regulation of distinct signaling pathways, and a member of the insulinase family has been implicated in propeptide processing.