Characterization of Immune Cells from Adipose Tissue.
Characterization of Immune Cells from Adipose Tissue.
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DOI：
10.1002/cpim.86
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发表时间：
2019-09-01
影响因子：
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通讯作者：
Zheng, Ye
中科院分区：
文献类型：
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作者：
Bapat, Sagar P;Liang, Yuqiong;Zheng, Ye
脂肪组织（AT）在维持机体代谢稳态中发挥着至关重要的作用。研究表明，AT 中含有多种协调和调节 AT 功能的免疫细胞。这种脂肪免疫系统是高度动态的，反映了有机体的生理状态（例如，肥胖、瘦、衰老或年轻）以及与禁食和进食节律相关的 AT 不断的生理重塑。 AT 的许多适应性和适应不良功能变化是通过不同的 AT 驻留免疫细胞组的数量和质量的变化来调节的。在这里，我们提出了基于流式细胞术的协议，通过构建脂肪驻留免疫细胞（巨噬细胞、树突状细胞、中性粒细胞、嗜酸性粒细胞、NK细胞、先天淋巴细胞、T细胞和B细胞等）普查来评估AT内免疫系统的动态状态，我们将其称为脂肪免疫谱（AIP）。构建 AIP 可以成为 AT 健康和功能评估的一个组成部分。本文介绍了生成此类 AIP 的协议。 © 2019 John Wiley & Sons, Inc. 版权所有
Adipose tissue (AT) serves a crucial role in maintaining organismal metabolic homeostasis. Studies have demonstrated that AT is populated with a diverse array of immune cells that coordinate and regulate AT function. This adipo-immune system is highly dynamic, reflecting the physiologic state of the organism (e.g., obese, lean, aged, or young) as well as the constant physiologic remodeling of AT associated with the daily rhythms of fasting and feeding. Many of the adaptive and maladaptive functional changes of AT are regulated by changes in the quantity and quality of distinct sets of AT-resident immune cells. Here we present protocols to assess the dynamic state of the immune system within AT by constructing censuses of adipose-resident immune cells (macrophages, dendritic cells, neutrophils, eosinophils, NK cells, innate lymphocytes, T cells, and B cells, etc.) based on flow cytometry, which we term adipo-immune profiles (AIPs). Constructing AIPs can be an integral part of assessment for AT health and function. This article describes the protocols to generate such AIPs. © 2019 by John Wiley & Sons, Inc.