Discrimination Between Normal and Cancerous Cells Using AFM.
Discrimination Between Normal and Cancerous Cells Using AFM.
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DOI：
10.1007/s12668-016-0191-3
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发表时间：
2016
期刊：
影响因子：
3
通讯作者：
Lekka M
中科院分区：
文献类型：
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作者：
Lekka M
目前，活细胞的生物力学成为人们关注的焦点，因为原子力显微镜 (AFM) 等技术具有显着的能力，可以直接在活细胞上探测单细胞水平的细胞特性。迄今为止进行的研究提供的数据一方面显示了细胞力学作为各种病理变化的生物标志物的用途，另一方面揭示了生物力学的相对性质。在 AFM 中，活细胞的弹性特性由压痕实验提供，并通过杨氏模量（此处定义为细胞变形能力的量度）进行定量描述。在此，总结并介绍了直接比较正常细胞和癌细胞机械性能的 AFM 研究，以及与杨氏模量相对性相关的一些重要问题。
Currently, biomechanics of living cells is in the focus of interest due to noticeable capability of such techniques like atomic force microscopy (AFM) to probe cellular properties at the single cell level directly on living cells. The research carried out, so far, delivered data showing, on the one hand, the use of cellular mechanics as a biomarker of various pathological changes, which, on the other hand, reveal relative nature of biomechanics. In the AFM, the elastic properties of living cells are delivered from indentation experiments and described quantitatively by Young’s modulus defined here as a measure of cellular deformability. Here, the AFM studies directly comparing the mechanical properties of normal and cancerous cells are summarized and presented together with a few important issues related to the relativeness of Young’s modulus.