Phenolic compounds, antiradical activity and antioxidant capacity of oil-bearing rose (Rosa damascena Mill.) extracts
Phenolic compounds, antiradical activity and antioxidant capacity of oil-bearing rose (Rosa damascena Mill.) extracts
复制标题
DOI：
10.1016/j.indcrop.2012.04.045
复制
发表时间：
2013-01-01
影响因子：
5.9
通讯作者：
Baydar, Hasan
中科院分区：
文献类型：
--
作者：
Baydar, Nilgun Gokturk;Baydar, Hasan
采用Folin-Ciocalteu法测定总酚含量，DMAC法测定总黄烷醇含量，Neu试剂法测定总黄酮醇含量，DPPH法测定抗自由基活性，FRAP法测定抗氧化能力，HPLC法测定总酚含量，和含油玫瑰的绿色叶子。样品经干燥和粉碎后，用甲醇进行热提取和冷提取。而热提取得到更多的提取物收率，冷提取得到更多的总酚，黄烷醇和黄酮醇含量。总酚含量以热、冷甲醇提取物最高，分别为478.34和530.40mgGAE/g。各种酚类化合物的相对浓度不受提取方法的显著影响。鲜花和花提取物中的没食子酸以及叶提取物中的（+）-儿茶素和（-）-表儿茶素是含量最丰富的酚类化合物。叶提取物在50 μ g/ml的浓度下表现出更多的抗自由基活性，甚至比合成抗氧化剂如Trolox、BHA和BHT更好。叶冷提取物也具有最强的抗氧化性能，当在700 nm处测量吸光度时，在100和150 μ g/ml的浓度下分别为1.43和1.72。最强的抗氧化性能与FRAP测定测定有叶冷提取物为1.43和1.72 μ g/ml的浓度为100和150 μ g/ml，分别。这些结果表明，含油玫瑰副产物，一个大规模的废物，可以评估为天然抗氧化剂源。(C)2012爱思唯尔有限公司版权所有。
The main target in the study was to determine total phenolic content by the Folin-Ciocalteu method, total flavanols by the DMAC method, total flavonols with Neu's reagent solution, antiradical activity by the DPPH method, antioxidant capacity by ferric reducing antioxidant power (FRAP) method and phenolic compounds by HPLC system were analyzed and measured in the extracts from the fresh and spent flowers, and green leaves of oil-bearing-rose (Rosa damascena Mill.). After drying and powdering of the samples, hot and cold extractions with methanol were made. While hot extractions gave more extract yields, cold extractions gave more total phenolic, flavanol and flavonol contents. The highest values of total phenolics were obtained from the hot and cold methanolic extractions of leaf with 478.34 and 530.40 mg GAE/g, respectively. The relative concentrations of the various phenolic compounds were not noteworthy influenced by the extraction methods. Gallic acid in the fresh and spent flower extracts, and (+)-catechin and (-)-epicatechin in the leaf extract were the most abundant phenolic compounds. Leaf extracts exhibited more antiradical activity at concentration of 50 mu g/ml even better than from synthetic antioxidants as Trolox, BHA and BHT. The leaf cold extractions had also the strongest antioxidant properties when measured with the absorbance was measured at 700 nm as 1.43 and 1.72 at the concentrations of 100 and 150 mu g/ml, respectively. The strongest antioxidant properties measured with the FRAP assay had leaf cold extractions as 1.43 and 1.72 mu g/ml at the concentrations of 100 and 150 mu g/ml, respectively. As a conclusion, these results indicate that oil-bearing rose by-products, a large scale wastes, can be evaluated for natural antioxidant sources. (C) 2012 Elsevier B.V. All rights reserved.